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10.1002/cpch.8 http://scihub22266oqcxt.onion/10.1002/cpch.8 C5183975!5183975!27622567     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27622567&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Curr+Protoc+Chem+Biol 2016 ; 8 (3): 147-78 Nephropedia Template TP {{tp|p=27622567|t=2016. Fabrication of 3D Reconstituted Organoid Arrays by DNA-programmed Assembly of Cells (DPAC) |pdf=|usr=}}{{27622567}} gab.com Text Fabrication of 3D Reconstituted Organoid Arrays by DNA-programmed Assembly of Cells (DPAC) JO: Curr Protoc Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=27622567 #FOAvip Tissues are the organizational units of function in metazoan organisms. Tissues comprise an assortment of cellular building blocks, soluble factors, and extracellular matrix (ECM) that are composed into specific three dimensional (3D) structures. The capacity to reconstitute tissues in vitro with the structural complexity observed in vivo is key to understanding processes such as morphogenesis, homeostasis, and disease. In this unit, we describe DNA-programmed Assembly of Cells (DPAC), a method to fabricate viable, functional arrays of organoid-like tissues within 3D ECM gels. In DPAC, dissociated cells are chemically functionalized with degradable oligonucleotide ?velcro,? allowing rapid, specific, and reversible cell adhesion to a two-dimensional (2D) template patterned with complementary DNA. An iterative assembly process builds up organoids, layer-by-layer, from this initial 2D template and into the third dimension. Cleavage of the DNA releases the completed array of tissues that are captured and fully embedded in ECM gels for culture and observation. DPAC controls the size, shape, composition, and spatial heterogeneity of organoids, and permits positioning constituent cells with single-cell resolution even within cultures several centimeters long. Twit Text FOAVip Fabrication of 3D Reconstituted Organoid Arrays by DNA-programmed Assembly of Cells (DPAC) ????Curr Protoc Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=27622567 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27622567 #FOAvip English Wikipedia <ref>{{Cite pmid|27622567}}</ref> Fabrication of 3D Reconstituted Organoid Arrays by DNA-programmed Assembly of Cells (DPAC) #MMPMID27622567 Todhunter ME; Weber RJ; Farlow J; Jee NY; Cerchiari AE; Gartner ZJ Curr Protoc Chem Biol 2016[Sep]; 8 (3): 147-78 PMID27622567show ga Tissues are the organizational units of function in metazoan organisms. Tissues comprise an assortment of cellular building blocks, soluble factors, and extracellular matrix (ECM) that are composed into specific three dimensional (3D) structures. The capacity to reconstitute tissues in vitro with the structural complexity observed in vivo is key to understanding processes such as morphogenesis, homeostasis, and disease. In this unit, we describe DNA-programmed Assembly of Cells (DPAC), a method to fabricate viable, functional arrays of organoid-like tissues within 3D ECM gels. In DPAC, dissociated cells are chemically functionalized with degradable oligonucleotide ?velcro,? allowing rapid, specific, and reversible cell adhesion to a two-dimensional (2D) template patterned with complementary DNA. An iterative assembly process builds up organoids, layer-by-layer, from this initial 2D template and into the third dimension. Cleavage of the DNA releases the completed array of tissues that are captured and fully embedded in ECM gels for culture and observation. DPAC controls the size, shape, composition, and spatial heterogeneity of organoids, and permits positioning constituent cells with single-cell resolution even within cultures several centimeters long. äFabrication of 3D Reconstituted Organoid Arrays by DNA-programmed Asse(epmc).pdf DeepDyve Pubget Overpricing