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10.1152/ajpendo.00515.2015 http://scihub22266oqcxt.onion/10.1152/ajpendo.00515.2015 C5130356!5130356 !27624103     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27624103 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Am+J+Physiol+Endocrinol+Metab 2016 ; 311 (5 ): E859-E868 Nephropedia Template TP {{tp|p=27624103 |t=2016. Development of a reliable automated screening system to identify small molecules and biologics that promote human ?-cell regeneration |pdf=|usr=}}{{27624103 }} gab.com Text Development of a reliable automated screening system to identify small molecules and biologics that promote human -cell regeneration JO: Am J Physiol Endocrinol Metab http://www.kidney.de/pget.php?&tw=1&pmid=27624103 #FOAvip Numerous compounds stimulate rodent ?-cell proliferation; however, translating these findings to human ?-cells remains a challenge. To examine human ?-cell proliferation in response to such compounds, we developed a medium-throughput in vitro method of quantifying adult human ?-cell proliferation markers. This method is based on high-content imaging of dispersed islet cells seeded in 384-well plates and automated cell counting that identifies fluorescently labeled ?-cells with high specificity using both nuclear and cytoplasmic markers. ?-Cells from each donor were assessed for their function and ability to enter the cell cycle by cotransduction with adenoviruses encoding cell cycle regulators cdk6 and cyclin D3. Using this approach, we tested 12 previously identified mitogens, including neurotransmitters, hormones, growth factors, and molecules, involved in adenosine and Tgf-1? signaling. Each compound was tested in a wide concentration range either in the presence of basal (5 mM) or high (11 mM) glucose. Treatment with the control compound harmine, a Dyrk1a inhibitor, led to a significant increase in Ki-67(+) ?-cells, whereas treatment with other compounds had limited to no effect on human ?-cell proliferation. This new scalable approach reduces the time and effort required for sensitive and specific evaluation of human ?-cell proliferation, thus allowing for increased testing of candidate human ?-cell mitogens. Twit Text FOAVip Development of a reliable automated screening system to identify small molecules and biologics that promote human -cell regeneration ????Am J Physiol Endocrinol Metab http://www.kidney.de/pget.php?&tw=1&pmid=27624103 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27624103 #FOAvip English Wikipedia <ref>{{Cite pmid|27624103 }}</ref> Development of a reliable automated screening system to identify small molecules and biologics that promote human ?-cell regeneration #MMPMID27624103 Aamodt KI ; Aramandla R ; Brown JJ ; Fiaschi-Taesch N ; Wang P ; Stewart AF ; Brissova M ; Powers AC Am J Physiol Endocrinol Metab 2016[Nov]; 311 (5 ): E859-E868 PMID27624103 show ga Numerous compounds stimulate rodent ?-cell proliferation; however, translating these findings to human ?-cells remains a challenge. To examine human ?-cell proliferation in response to such compounds, we developed a medium-throughput in vitro method of quantifying adult human ?-cell proliferation markers. This method is based on high-content imaging of dispersed islet cells seeded in 384-well plates and automated cell counting that identifies fluorescently labeled ?-cells with high specificity using both nuclear and cytoplasmic markers. ?-Cells from each donor were assessed for their function and ability to enter the cell cycle by cotransduction with adenoviruses encoding cell cycle regulators cdk6 and cyclin D3. Using this approach, we tested 12 previously identified mitogens, including neurotransmitters, hormones, growth factors, and molecules, involved in adenosine and Tgf-1? signaling. Each compound was tested in a wide concentration range either in the presence of basal (5 mM) or high (11 mM) glucose. Treatment with the control compound harmine, a Dyrk1a inhibitor, led to a significant increase in Ki-67(+) ?-cells, whereas treatment with other compounds had limited to no effect on human ?-cell proliferation. This new scalable approach reduces the time and effort required for sensitive and specific evaluation of human ?-cell proliferation, thus allowing for increased testing of candidate human ?-cell mitogens. |Activins/pharmacology [MESH] |Adenosine A2 Receptor Agonists/pharmacology [MESH] |Adenosine-5'-(N-ethylcarboxamide)/pharmacology [MESH] |Adenosine/analogs & derivatives/pharmacology [MESH] |Adult [MESH] |Automation [MESH] |Cell Culture Techniques [MESH] |Cell Proliferation/*drug effects [MESH] |Drug Evaluation, Preclinical [MESH] |Erythropoietin/pharmacology [MESH] |Exenatide [MESH] |Female [MESH] |GABA Agents/pharmacology [MESH] |Harmine/pharmacology [MESH] |Humans [MESH] |Incretins/pharmacology [MESH] |Insulin-Secreting Cells/*drug effects [MESH] |Male [MESH] |Middle Aged [MESH] |Monoamine Oxidase Inhibitors/pharmacology [MESH] |Myostatin/pharmacology [MESH] |Nucleosides/pharmacology [MESH] |Peptides/pharmacology [MESH] |Platelet-Derived Growth Factor/pharmacology [MESH] |Prolactin/pharmacology [MESH] |Regeneration/drug effects [MESH] |Serotonin Receptor Agonists/pharmacology [MESH] |Serotonin/pharmacology [MESH] |Vasodilator Agents/pharmacology [MESH] |Venoms/pharmacology [MESH] |Young Adult [MESH]Development of a reliable automated screening system to identify small(epmc).pdf DeepDyve Pubget Overpricing