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10.1002/1873-3468.12388 http://scihub22266oqcxt.onion/10.1002/1873-3468.12388 C5129459!5129459 !27597178     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27597178 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       FEBS+Lett 2016 ; 590 (19 ): 3416-3424 Nephropedia Template TP {{tp|p=27597178 |t=2016. Efficient generation of selection-gene-free rat knockout models by homologous recombination in ES cells |pdf=|usr=}}{{27597178 }} gab.com Text Efficient generation of selection-gene-free rat knockout models by homologous recombination in ES cells JO: FEBS Lett http://www.kidney.de/pget.php?&tw=1&pmid=27597178 #FOAvip Embryonic stem cell (ES cell)-based rat knockout technology, although successfully developed in 2010, has seen very limited usage to date due to low targeting efficiency and a lack of optimized procedures. In this study, we performed gene targeting in ES cells from the Sprague-Dawley (SD) and the Fischer 344 (F344) rat strains using an optimized procedure and the self-excising neomycin (neo)-positive selection cassette ACN to successfully generate Leptin and Trp53 knockout rats that did not carry the selection gene. These results demonstrate that our simplified targeting strategy using ACN provides an efficient approach to knock out many other rat genes. Twit Text FOAVip Efficient generation of selection-gene-free rat knockout models by homologous recombination in ES cells ????FEBS Lett http://www.kidney.de/pget.php?&tw=1&pmid=27597178 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27597178 #FOAvip English Wikipedia <ref>{{Cite pmid|27597178 }}</ref> Efficient generation of selection-gene-free rat knockout models by homologous recombination in ES cells #MMPMID27597178 Lan H ; Li S ; Guo Z ; Men H ; Wu Y ; Li N ; Bryda EC ; Capecchi MR ; Wu S FEBS Lett 2016[Oct]; 590 (19 ): 3416-3424 PMID27597178 show ga Embryonic stem cell (ES cell)-based rat knockout technology, although successfully developed in 2010, has seen very limited usage to date due to low targeting efficiency and a lack of optimized procedures. In this study, we performed gene targeting in ES cells from the Sprague-Dawley (SD) and the Fischer 344 (F344) rat strains using an optimized procedure and the self-excising neomycin (neo)-positive selection cassette ACN to successfully generate Leptin and Trp53 knockout rats that did not carry the selection gene. These results demonstrate that our simplified targeting strategy using ACN provides an efficient approach to knock out many other rat genes. |*Homologous Recombination [MESH] |Animals [MESH] |Cell Line [MESH] |Embryonic Stem Cells/*cytology/metabolism [MESH] |Gene Knockout Techniques/*methods [MESH] |Leptin/genetics/metabolism [MESH] |Male [MESH] |Rats [MESH] |Rats, Inbred F344 [MESH] |Rats, Sprague-Dawley [MESH]Efficient generation of selection-gene-free rat knockout models by hom(epmc).pdf DeepDyve Pubget Overpricing