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2016 ; 54
(5
): 605-616
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Assessing the Archaeoparasitological Potential of Quids As a Source Material for
Immunodiagnostic Analyses
#MMPMID27853117
Morrow JJ
; Reinhard KJ
Korean J Parasitol
2016[Oct]; 54
(5
): 605-616
PMID27853117
show ga
In the present study, quids from La Cueva de los Muertos Chiquitos (CMC) were
subjected to ELISA tests for 2 protozoan parasites, Toxoplasma gondii (n=45) and
Trypanosoma cruzi (n=43). The people who occupied CMC, the Loma San Gabriel,
lived throughout much of present-day Durango and Zacatecas in Mexico. The known
pathoecology of these people puts them into at-risk categories for the
transmission of T. gondii and T. cruzi. Human antibodies created in response to
these 2 parasites can be detected in modern saliva using ELISA kits intended for
use with human serum. For these reasons, quids were reconstituted and subjected
to ELISA testing. All test wells yielded negative results. These results could be
a factor of improper methods because there is no precedence for this work in the
existing literature. The results could equally be a simple matter of parasite
absence among those people who occupied CMC. A final consideration is the
taphonomy of human antibodies and whether or not ELISA is a sufficient method for
recovering antibodies from archaeological contexts. An additional ELISA test
targeting secretory IgA (sIgA) was conducted to further examine the failure to
detect parasite-induced antibodies from quids. Herein, the methods used for quid
preparation and ELISA procedures are described so that they can be further
developed by future researchers. The results are discussed in light of the
potential future of quid analysis.