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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Aesthet+Surg+J
2016 ; 36
(3
): 349-57
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Preparation, Characterization, and Clinical Implications of Human Decellularized
Adipose Tissue Extracellular Matrix (hDAM): A Comprehensive Review
#MMPMID26333991
Banyard DA
; Borad V
; Amezcua E
; Wirth GA
; Evans GR
; Widgerow AD
Aesthet Surg J
2016[Mar]; 36
(3
): 349-57
PMID26333991
show ga
Fat grafting is commonly employed by plastic and reconstructive surgeons to
address contour abnormalities and soft-tissue defects; however, because retention
rates and thus volume filling effects are unpredictable, there is a search for
new and innovative approaches. Initial studies on the use of human decellularized
adipose tissue extracellular matrix (hDAM) show promise for its use not only in
tissue engineering, but also in fat grafting. In this review, we examine and
analyze the literature for the preparation, characterization, and use of hDAM and
its derivatives in tissue engineering and plastic surgery applications. All
studies reviewed involve physical, chemical, and/or biological treatment stages
for the preparation of hDAM; however a distinction should be made between
detergent and nondetergent-based processing, the latter of which appears to
preserve the native integrity of the hDAM while most-efficiently achieving
complete decellularization. Methods of hDAM characterization vary among groups
and included simple and immunohistochemical staining, biochemical assays,
3-dimensional (3D) imaging, and mechano-stress testing, all of which are
necessary to achieve a comprehensive description of this novel tissue. Finally,
we examine the various preclinical models utilized to optimize hDAM performance,
which primarily include the addition of adipose-derived stem cells or
cross-linking agents. Overall, hDAM appears to be a promising adjunct in
fat-grafting applications or even possibly as a stand-alone soft-tissue filler
with off-the-shelf potential for commercial applications.
|*Cosmetic Techniques/adverse effects
[MESH]
|Adipose Tissue/*transplantation
[MESH]
|Extracellular Matrix/*transplantation
[MESH]
|Humans
[MESH]
|Plastic Surgery Procedures/adverse effects/*methods
[MESH]
|Postoperative Complications/etiology
[MESH]
|Tissue and Organ Harvesting/adverse effects/*methods
[MESH]