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10.1016/j.chembiol.2016.10.006 http://scihub22266oqcxt.onion/10.1016/j.chembiol.2016.10.006 C5127401!5127401!27818300     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Chem+Biol 2016 ; 23 (11): 1417-27 Nephropedia Template TP {{tp|p=27818300|t=2016. Multicolor electron microscopy for simultaneous visualization of multiple molecular species |pdf=|usr=}}{{27818300}} gab.com Text Multicolor electron microscopy for simultaneous visualization of multiple molecular species JO: Cell Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=27818300 #FOAvip Electron microscopy (EM) remains the primary method for imaging cellular and tissue ultrastructure, although simultaneous localization of multiple specific molecules continues to be a challenge for EM. We present a method for obtaining multicolor EM views of multiple subcellular components. The method uses sequential, localized deposition of different lanthanides by photosensitizers, small molecule probes or peroxidases. Detailed view of biological structures is created by overlaying conventional electron micrographs with pseudocolor lanthanide elemental maps derived from distinctive electron energy-loss spectra (EELS) of each lanthanide deposit via energy-filtered transmission electron microscopy (EFTEM). This results in multicolor EM images analogous to multicolor fluorescence but with the benefit of the full spatial resolution of EM. We illustrate the power of this methodology by visualizing hippocampal astrocytes to show that processes from two astrocytes can share a single synapse. We also show that polyarginine-based cell-penetrating peptides enter the cell via endocytosis, and that newly synthesized PKM? in cultured neurons preferentially localize to the post-synaptic membrane. Twit Text FOAVip Multicolor electron microscopy for simultaneous visualization of multiple molecular species ????Cell Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=27818300 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27818300 #FOAvip English Wikipedia <ref>{{Cite pmid|27818300}}</ref> Multicolor electron microscopy for simultaneous visualization of multiple molecular species #MMPMID27818300 Adams SR; Mackey MR; Ramachandra R; Palida Lemieux SF; Steinbach P; Bushong EA; Butko MT; Giepmans BN; Ellisman MH; Tsien RY Cell Chem Biol 2016[Nov]; 23 (11): 1417-27 PMID27818300show ga Electron microscopy (EM) remains the primary method for imaging cellular and tissue ultrastructure, although simultaneous localization of multiple specific molecules continues to be a challenge for EM. We present a method for obtaining multicolor EM views of multiple subcellular components. The method uses sequential, localized deposition of different lanthanides by photosensitizers, small molecule probes or peroxidases. Detailed view of biological structures is created by overlaying conventional electron micrographs with pseudocolor lanthanide elemental maps derived from distinctive electron energy-loss spectra (EELS) of each lanthanide deposit via energy-filtered transmission electron microscopy (EFTEM). This results in multicolor EM images analogous to multicolor fluorescence but with the benefit of the full spatial resolution of EM. We illustrate the power of this methodology by visualizing hippocampal astrocytes to show that processes from two astrocytes can share a single synapse. We also show that polyarginine-based cell-penetrating peptides enter the cell via endocytosis, and that newly synthesized PKM? in cultured neurons preferentially localize to the post-synaptic membrane. äMulticolor electron microscopy for simultaneous visualization of multi(epmc).pdf DeepDyve Pubget Overpricing