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10.1038/ncomms13631 http://scihub22266oqcxt.onion/10.1038/ncomms13631 C5123018!5123018 !27876798     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27876798 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Commun 2016 ; 7 (ä): 13631 Nephropedia Template TP {{tp|p=27876798 |t=2016. Proliferation of Listeria monocytogenes L-form cells by formation of internal and external vesicles |pdf=|usr=}}{{27876798 }} gab.com Text Proliferation of Listeria monocytogenes L-form cells by formation of internal and external vesicles JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=27876798 #FOAvip L-forms are cell wall-deficient bacteria that divide through unusual mechanisms, involving dynamic perturbations of the cellular shape and generation of vesicles, independently of the cell-division protein FtsZ. Here we describe FtsZ-independent mechanisms, involving internal and external vesicles, by which Listeria monocytogenes L-forms proliferate. Using micromanipulation of single cells and vesicles, we show that small vesicles are formed by invagination within larger intracellular vesicles, receive cytoplasmic content, and represent viable progeny. In addition, the L-forms can reproduce by pearling, that is, generation of extracellular vesicles that remain transiently linked to their mother cell via elastic membranous tubes. Using photobleaching and fluorescence recovery, we demonstrate cytoplasmic continuity and transfer through these membranous tubes. Our findings indicate that L-forms' polyploidy and extended interconnectivity through membranous tubes contribute to the generation of viable progeny independently of dedicated division machinery, and further support L-forms as models for studies of potential multiplication mechanisms of hypothetical primitive cells. Twit Text FOAVip Proliferation of Listeria monocytogenes L-form cells by formation of internal and external vesicles ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=27876798 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27876798 #FOAvip English Wikipedia <ref>{{Cite pmid|27876798 }}</ref> Proliferation of Listeria monocytogenes L-form cells by formation of internal and external vesicles #MMPMID27876798 Studer P ; Staubli T ; Wieser N ; Wolf P ; Schuppler M ; Loessner MJ Nat Commun 2016[Nov]; 7 (ä): 13631 PMID27876798 show ga L-forms are cell wall-deficient bacteria that divide through unusual mechanisms, involving dynamic perturbations of the cellular shape and generation of vesicles, independently of the cell-division protein FtsZ. Here we describe FtsZ-independent mechanisms, involving internal and external vesicles, by which Listeria monocytogenes L-forms proliferate. Using micromanipulation of single cells and vesicles, we show that small vesicles are formed by invagination within larger intracellular vesicles, receive cytoplasmic content, and represent viable progeny. In addition, the L-forms can reproduce by pearling, that is, generation of extracellular vesicles that remain transiently linked to their mother cell via elastic membranous tubes. Using photobleaching and fluorescence recovery, we demonstrate cytoplasmic continuity and transfer through these membranous tubes. Our findings indicate that L-forms' polyploidy and extended interconnectivity through membranous tubes contribute to the generation of viable progeny independently of dedicated division machinery, and further support L-forms as models for studies of potential multiplication mechanisms of hypothetical primitive cells. |Bacterial Proteins/genetics/metabolism [MESH] |Cell Division/*physiology [MESH] |Cell Wall/physiology [MESH] |Cytoplasmic Vesicles/*physiology [MESH] |Gene Expression Regulation, Bacterial/physiology [MESH] |Listeria monocytogenes/*physiology [MESH]Proliferation of Listeria monocytogenes L-form cells by formation of i(epmc).pdf DeepDyve Pubget Overpricing