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10.1080/19491034.2016.1239000 http://scihub22266oqcxt.onion/10.1080/19491034.2016.1239000 C5120596!5120596!27676121     free     free     free       Nucleus 2016 ; 7 (5): 476-84 Nephropedia Template TP {{tp|p=27676121|t=2016. Determination of local chromatin composition by CasID |pdf=|usr=}}{{27676121}} gab.com Text Determination of local chromatin composition by CasID JO: Nucleus http://www.kidney.de/pget.php?&tw=1&pmid=27676121 #FOAvip Chromatin structure and function are determined by a plethora of proteins whose genome-wide distribution is typically assessed by immunoprecipitation (ChIP). Here, we developed a novel tool to investigate the local chromatin environment at specific DNA sequences. We combined the programmable DNA binding of dCas9 with the promiscuous biotin ligase BirA* (CasID) to biotinylate proteins in the direct vicinity of specific loci. Subsequent streptavidin-mediated precipitation and mass spectrometry identified both known and previously unknown chromatin factors associated with repetitive telomeric, major satellite and minor satellite DNA. With super-resolution microscopy, we confirmed the localization of the putative transcription factor ZNF512 at chromocenters. The versatility of CasID facilitates the systematic elucidation of functional protein complexes and locus-specific chromatin composition. Twit Text FOAVip Determination of local chromatin composition by CasID ????Nucleus http://www.kidney.de/pget.php?&tw=1&pmid=27676121 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27676121 #FOAvip English Wikipedia <ref>{{Cite pmid|27676121}}</ref> Determination of local chromatin composition by CasID #MMPMID27676121 Schmidtmann E; Anton T; Rombaut P; Herzog F; Leonhardt H Nucleus 2016[]; 7 (5): 476-84 PMID27676121show ga Chromatin structure and function are determined by a plethora of proteins whose genome-wide distribution is typically assessed by immunoprecipitation (ChIP). Here, we developed a novel tool to investigate the local chromatin environment at specific DNA sequences. We combined the programmable DNA binding of dCas9 with the promiscuous biotin ligase BirA* (CasID) to biotinylate proteins in the direct vicinity of specific loci. Subsequent streptavidin-mediated precipitation and mass spectrometry identified both known and previously unknown chromatin factors associated with repetitive telomeric, major satellite and minor satellite DNA. With super-resolution microscopy, we confirmed the localization of the putative transcription factor ZNF512 at chromocenters. The versatility of CasID facilitates the systematic elucidation of functional protein complexes and locus-specific chromatin composition. äDetermination of local chromatin composition by CasID (epmc).pdf DeepDyve Pubget Overpricing