Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27877211
&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215
Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27877211
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 J+Cancer
2016 ; 7
(14
): 1960-1967
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
The Three-Gene Signature in Urinary Extracellular Vesicles from Patients with
Clear Cell Renal Cell Carcinoma
#MMPMID27877211
De Palma G
; Sallustio F
; Curci C
; Galleggiante V
; Rutigliano M
; Serino G
; Ditonno P
; Battaglia M
; Schena FP
J Cancer
2016[]; 7
(14
): 1960-1967
PMID27877211
show ga
Renal cell carcinoma (RCC) accounts for more than 2% of neoplasias in humans
worldwide. Renal biopsy is the gold standard among the diagnostic procedures, but
it is invasive and not suitable for all patients. Therefore, new reliable and
non-invasive biomarkers for RCC are required. Secretion of extracellular vesicles
(EVs), containing RNA molecules that can be transferred between cells, appears to
be a common feature of neoplasia. Consistently, cancer-derived EVs are increased
in blood and urine. Therefore, urinary samples may be a non-invasive approach for
discovering new diagnostic biomarkers. We enrolled 46 patients of whom 33 with
clear cell renal cell carcinoma (ccRCC) and 22 healthy subjects (HS). Urinary EVs
were isolated by differential centrifugation. Microarray analysis led to the
identification of RNA molecules that were validated using RT-qPCR. We found that
urinary exosomal shuttle RNA (esRNA) pattern was significantly different in ccRCC
patients compared to HS and to non-clear cell RCC (non-ccRCC) and we identified
three esRNAs involved in the tumor biology that may be potentially suitable as
non-invasive gene signature. GSTA1, CEBPA and PCBD1 esRNA levels were decreased
in urine of patients compared with HS. This pattern was specific of the ccRCC and
one month after partial or radical nephrectomy the esRNA levels increased to
reach the normal level. This study suggests, for the first time, the potential
use of the RNA content of urinary EVs to provide a non-invasive first step to
diagnose the ccRCC.
free
free
free
