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10.1186/s12885-016-2879-8 http://scihub22266oqcxt.onion/10.1186/s12885-016-2879-8 C5114842!5114842 !27855654     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27855654 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       BMC+Cancer 2016 ; 16 (1 ): 896 Nephropedia Template TP {{tp|p=27855654 |t=2016. Salinomycin inhibits metastatic colorectal cancer growth and interferes with Wnt/?-catenin signaling in CD133(+) human colorectal cancer cells |pdf=|usr=}}{{27855654 }} gab.com Text Salinomycin inhibits metastatic colorectal cancer growth and interferes with Wnt/ -catenin signaling in CD133(+) human colorectal cancer cells JO: BMC Cancer http://www.kidney.de/pget.php?&tw=1&pmid=27855654 #FOAvip BACKGROUND: The polyether antibiotic Salinomycin (Sal) is regarded as an inhibitor of cancer stem cells. Its effectiveness on human colorectal cancer (CRC) cells in vitro has been demonstrated before. The aim of this study was to establish a murine model to investigate the effectiveness of Sal in vivo. Furthermore, we investigated the impact of Sal on Wnt/?-catenin signaling in human CD133(+) CRC cells. METHODS: The two murine CRC cell lines MC38 and CT26 were used to analyze the impact of Sal on tumor cell proliferation, viability, migration, cell cycle progression and cell death in vitro. For in vivo studies, CT26 cells were injected into syngeneic BALB/c mice to initiate (i) subcutaneous, (ii) orthotopic, or (iii) metastatic CRC growth. Sal was administered daily, 5-Fluoruracil served as a control. For mechanistic studies, the CD133(+)and CD133(-) subpopulations of human CRC cells were separated by flow cytometry and separately exposed to increasing concentrations of Sal. The impact on Wnt/?-catenin signaling was determined by Western blotting and quantitative PCR. RESULTS: Sal markedly impaired tumor cell viability, proliferation and migration, and induced necrotic cell death in vitro. CRC growth in vivo was likewise inhibited upon Sal treatment. Interference with Wnt signaling and reduced expression of the Wnt target genes Fibronectin and Lgr5 indicates a novel molecular mechanism, mediating anti-tumoral effects of Sal in CRC. CONCLUSION: Sal effectively impairs CRC growth in vivo. Furthermore, Sal acts as an inhibitor of Wnt/?-catenin signaling. Thus, Salinomycin represents a promising candidate for clinical CRC treatment. Twit Text FOAVip Salinomycin inhibits metastatic colorectal cancer growth and interferes with Wnt/ -catenin signaling in CD133(+) human colorectal cancer cells ????BMC Cancer http://www.kidney.de/pget.php?&tw=1&pmid=27855654 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27855654 #FOAvip English Wikipedia <ref>{{Cite pmid|27855654 }}</ref> Salinomycin inhibits metastatic colorectal cancer growth and interferes with Wnt/?-catenin signaling in CD133(+) human colorectal cancer cells #MMPMID27855654 Klose J ; Eissele J ; Volz C ; Schmitt S ; Ritter A ; Ying S ; Schmidt T ; Heger U ; Schneider M ; Ulrich A BMC Cancer 2016[Nov]; 16 (1 ): 896 PMID27855654 show ga BACKGROUND: The polyether antibiotic Salinomycin (Sal) is regarded as an inhibitor of cancer stem cells. Its effectiveness on human colorectal cancer (CRC) cells in vitro has been demonstrated before. The aim of this study was to establish a murine model to investigate the effectiveness of Sal in vivo. Furthermore, we investigated the impact of Sal on Wnt/?-catenin signaling in human CD133(+) CRC cells. METHODS: The two murine CRC cell lines MC38 and CT26 were used to analyze the impact of Sal on tumor cell proliferation, viability, migration, cell cycle progression and cell death in vitro. For in vivo studies, CT26 cells were injected into syngeneic BALB/c mice to initiate (i) subcutaneous, (ii) orthotopic, or (iii) metastatic CRC growth. Sal was administered daily, 5-Fluoruracil served as a control. For mechanistic studies, the CD133(+)and CD133(-) subpopulations of human CRC cells were separated by flow cytometry and separately exposed to increasing concentrations of Sal. The impact on Wnt/?-catenin signaling was determined by Western blotting and quantitative PCR. RESULTS: Sal markedly impaired tumor cell viability, proliferation and migration, and induced necrotic cell death in vitro. CRC growth in vivo was likewise inhibited upon Sal treatment. Interference with Wnt signaling and reduced expression of the Wnt target genes Fibronectin and Lgr5 indicates a novel molecular mechanism, mediating anti-tumoral effects of Sal in CRC. CONCLUSION: Sal effectively impairs CRC growth in vivo. Furthermore, Sal acts as an inhibitor of Wnt/?-catenin signaling. Thus, Salinomycin represents a promising candidate for clinical CRC treatment. |AC133 Antigen/*metabolism [MESH] |Animals [MESH] |Cell Line, Tumor [MESH] |Cell Movement/drug effects [MESH] |Cell Proliferation/drug effects [MESH] |Cell Survival/drug effects [MESH] |Colorectal Neoplasms/*drug therapy/metabolism [MESH] |Gene Expression Regulation, Neoplastic/drug effects [MESH] |Humans [MESH] |Mice [MESH] |Mice, Inbred BALB C [MESH] |Neoplasm Metastasis [MESH] |Pyrans/*administration & dosage/pharmacology [MESH] |Wnt Signaling Pathway/*drug effects [MESH]Salinomycin inhibits metastatic colorectal cancer growth and interfere(epmc).pdf DeepDyve Pubget Overpricing