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10.1038/srep36736 http://scihub22266oqcxt.onion/10.1038/srep36736 C5113122!5113122!27853247     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2016 ; 6 (ä): ä Nephropedia Template TP {{tp|p=27853247|t=2016. Six2creFrs2? knockout mice are a novel model of renal cystogenesis |pdf=|usr=}}{{27853247}} gab.com Text Six2creFrs2 knockout mice are a novel model of renal cystogenesis JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27853247 #FOAvip Six2cre-mediated deletion of Frs2? (Six2creFrs2?KO), a major fibroblast growth factor receptor (Fgfr) docking protein in mouse nephron progenitors results in perinatal renal hypoplasia; however, postnatal Six2creFrs2?KO kidneys develop cysts. We sought to determine the pathogenesis of Six2creFrs2?KO cyst formation. We performed histological assays, Western blots, and quantitative PCR (qPCR). While embryonic day (E) 18.5 Six2Frs2?KO kidneys were hypoplastic and not cystic, postnatal day (P) 7 mutants had proximal tubular-derived cysts that nearly replaced the renal parenchyma by P21. Mutants had high proximal tubular proliferation rates and interstitial fibrosis, similar to known polycystic kidney disease (PKD) models. Six2creFrs2?KO kidneys also had upregulation of Wnt/?catenin signaling, macrophage infiltration and chemokine production (e.g. ectopic Ccl2 in non-dilated proximal tubules), and augmented hedgehog signaling, features also seen in other PKD models. We saw increased Gli1 (hedgehog readout) in postnatal Six2creFrs2?KO interstitium and ectopic sonic hedgehog (Shh) in subsets of non-dilated P7 mutant proximal tubules (likely driving the stromal Gli expression). As ectopic tubular Shh and Ccl2 expression is seen after acute kidney injury (AKI), we interrogated another bone fide AKI marker, Kim1 and noted ectopic expression in P7 non-dilated proximal tubules. These observations suggest that aberrantly activated ?AKI? pathways may drive pathogenesis in PKD. Twit Text FOAVip Six2creFrs2 knockout mice are a novel model of renal cystogenesis ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27853247 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27853247 #FOAvip English Wikipedia <ref>{{Cite pmid|27853247}}</ref> Six2creFrs2? knockout mice are a novel model of renal cystogenesis #MMPMID27853247 Puri P; Bushnell D; Schaefer CM; Bates CM Sci Rep 2016[]; 6 (ä): ä PMID27853247show ga Six2cre-mediated deletion of Frs2? (Six2creFrs2?KO), a major fibroblast growth factor receptor (Fgfr) docking protein in mouse nephron progenitors results in perinatal renal hypoplasia; however, postnatal Six2creFrs2?KO kidneys develop cysts. We sought to determine the pathogenesis of Six2creFrs2?KO cyst formation. We performed histological assays, Western blots, and quantitative PCR (qPCR). While embryonic day (E) 18.5 Six2Frs2?KO kidneys were hypoplastic and not cystic, postnatal day (P) 7 mutants had proximal tubular-derived cysts that nearly replaced the renal parenchyma by P21. Mutants had high proximal tubular proliferation rates and interstitial fibrosis, similar to known polycystic kidney disease (PKD) models. Six2creFrs2?KO kidneys also had upregulation of Wnt/?catenin signaling, macrophage infiltration and chemokine production (e.g. ectopic Ccl2 in non-dilated proximal tubules), and augmented hedgehog signaling, features also seen in other PKD models. We saw increased Gli1 (hedgehog readout) in postnatal Six2creFrs2?KO interstitium and ectopic sonic hedgehog (Shh) in subsets of non-dilated P7 mutant proximal tubules (likely driving the stromal Gli expression). As ectopic tubular Shh and Ccl2 expression is seen after acute kidney injury (AKI), we interrogated another bone fide AKI marker, Kim1 and noted ectopic expression in P7 non-dilated proximal tubules. These observations suggest that aberrantly activated ?AKI? pathways may drive pathogenesis in PKD. äSix2creFrs2? knockout mice are a novel model of renal cystogenesis (epmc).pdf DeepDyve Pubget Overpricing