Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1016/j.molcel.2016.07.027 http://scihub22266oqcxt.onion/10.1016/j.molcel.2016.07.027 C5111854!5111854!27588603     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Cell 2016 ; 63 (5): 840-51 Nephropedia Template TP {{tp|p=27588603|t=2016. DNA Targeting by a Minimal CRISPR RNA-Guided Cascade |pdf=|usr=}}{{27588603}} gab.com Text DNA Targeting by a Minimal CRISPR RNA-Guided Cascade JO: Mol Cell http://www.kidney.de/pget.php?&tw=1&pmid=27588603 #FOAvip Bacteria employ surveillance complexes guided by CRISPR (clustered, regularly interspaced, short palindromic repeats) RNAs (crRNAs) to target foreign nucleic acids for destruction. Although most type I and type III CRISPR systems require four or more distinct proteins to form multi-subunit surveillance complexes, the type I-C systems use just three proteins to achieve crRNA maturation and double-stranded DNA target recognition. We show that each protein plays multiple functional and structural roles: Cas5c cleaves pre-crRNAs and recruits Cas7 to position the RNA guide for DNA binding and unwinding by Cas8c. Cryoelectron microscopy reconstructions of free and DNA-bound forms of the Cascade/I-C surveillance complex reveal conformational changes that enable R-loop formation with distinct positioning of each DNA strand. This streamlined type I-C system explains how CRISPR pathways can evolve compact structures that retain full functionality as RNA-guided DNA capture platforms. Twit Text FOAVip DNA Targeting by a Minimal CRISPR RNA-Guided Cascade ????Mol Cell http://www.kidney.de/pget.php?&tw=1&pmid=27588603 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27588603 #FOAvip English Wikipedia <ref>{{Cite pmid|27588603}}</ref> DNA Targeting by a Minimal CRISPR RNA-Guided Cascade #MMPMID27588603 Hochstrasser ML; Taylor DW; Kornfeld JE; Nogales E; Doudna JA Mol Cell 2016[Sep]; 63 (5): 840-51 PMID27588603show ga Bacteria employ surveillance complexes guided by CRISPR (clustered, regularly interspaced, short palindromic repeats) RNAs (crRNAs) to target foreign nucleic acids for destruction. Although most type I and type III CRISPR systems require four or more distinct proteins to form multi-subunit surveillance complexes, the type I-C systems use just three proteins to achieve crRNA maturation and double-stranded DNA target recognition. We show that each protein plays multiple functional and structural roles: Cas5c cleaves pre-crRNAs and recruits Cas7 to position the RNA guide for DNA binding and unwinding by Cas8c. Cryoelectron microscopy reconstructions of free and DNA-bound forms of the Cascade/I-C surveillance complex reveal conformational changes that enable R-loop formation with distinct positioning of each DNA strand. This streamlined type I-C system explains how CRISPR pathways can evolve compact structures that retain full functionality as RNA-guided DNA capture platforms. äDNA Targeting by a Minimal CRISPR RNA-Guided Cascade (epmc).pdf DeepDyve Pubget Overpricing