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10.1002/humu.23051 http://scihub22266oqcxt.onion/10.1002/humu.23051 C5111608!5111608!27406789     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27406789&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Hum+Mutat 2016 ; 37 (12): 1363-7 Nephropedia Template TP {{tp|p=27406789|t=2016. Fourth Generation of Next?Generation Sequencing Technologies: Promise and Consequences |pdf=|usr=}}{{27406789}} gab.com Text Fourth Generation of Next Generation Sequencing Technologies: Promise and Consequences JO: Hum Mutat http://www.kidney.de/pget.php?&tw=1&pmid=27406789 #FOAvip In this review, we discuss the emergence of the fourth?generation sequencing technologies that preserve the spatial coordinates of RNA and DNA sequences with up to subcellular resolution, thus enabling back mapping of sequencing reads to the original histological context. This information is used, for example, in two current large?scale projects that aim to unravel the function of the brain. Also in cancer research, fourth?generation sequencing has the potential to revolutionize the field. Cancer Research UK has named ?Mapping the molecular and cellular tumor microenvironment in order to define new targets for therapy and prognosis? one of the grand challenges in tumor biology. We discuss the advantages of sequencing nucleic acids directly in fixed cells over traditional next?generation sequencing (NGS) methods, the limitations and challenges that these new methods have to face to become broadly applicable, and the impact that the information generated by the combination of in situ sequencing and NGS methods will have in research and diagnostics. Twit Text FOAVip Fourth Generation of Next Generation Sequencing Technologies: Promise and Consequences ????Hum Mutat http://www.kidney.de/pget.php?&tw=1&pmid=27406789 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27406789 #FOAvip English Wikipedia <ref>{{Cite pmid|27406789}}</ref> Fourth Generation of Next?Generation Sequencing Technologies: Promise and Consequences #MMPMID27406789 Ke R; Mignardi M; Hauling T; Nilsson M Hum Mutat 2016[Dec]; 37 (12): 1363-7 PMID27406789show ga In this review, we discuss the emergence of the fourth?generation sequencing technologies that preserve the spatial coordinates of RNA and DNA sequences with up to subcellular resolution, thus enabling back mapping of sequencing reads to the original histological context. This information is used, for example, in two current large?scale projects that aim to unravel the function of the brain. Also in cancer research, fourth?generation sequencing has the potential to revolutionize the field. Cancer Research UK has named ?Mapping the molecular and cellular tumor microenvironment in order to define new targets for therapy and prognosis? one of the grand challenges in tumor biology. We discuss the advantages of sequencing nucleic acids directly in fixed cells over traditional next?generation sequencing (NGS) methods, the limitations and challenges that these new methods have to face to become broadly applicable, and the impact that the information generated by the combination of in situ sequencing and NGS methods will have in research and diagnostics. äFourth Generation of Next?Generation Sequencing Technologies: Promise (epmc).pdf DeepDyve Pubget Overpricing