Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1016/j.celrep.2016.09.002 http://scihub22266oqcxt.onion/10.1016/j.celrep.2016.09.002 C5111363!5111363 !27681417     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27681417 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27681417 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Cell+Rep 2016 ; 17 (1 ): 19-28 Nephropedia Template TP {{tp|p=27681417 |t=2016. Super-Enhancers at the Nanog Locus Differentially Regulate Neighboring Pluripotency-Associated Genes |pdf=|usr=}}{{27681417 }} gab.com Text Super-Enhancers at the Nanog Locus Differentially Regulate Neighboring Pluripotency-Associated Genes JO: Cell Rep http://www.kidney.de/pget.php?&tw=1&pmid=27681417 #FOAvip Super-enhancers are tissue-specific cis-regulatory elements that drive expression of genes associated with cell identity and malignancy. A cardinal feature of super-enhancers is that they are transcribed to produce enhancer-derived RNAs (eRNAs). It remains unclear whether super-enhancers robustly activate genes in situ and whether their functions are attributable to eRNAs or the DNA element. CRISPR/Cas9 was used to systematically delete three discrete super-enhancers at the Nanog locus in embryonic stem cells, revealing functional differences in Nanog transcriptional regulation. One distal super-enhancer 45 kb upstream of Nanog (-45 enhancer) regulates both nearest neighbor genes, Nanog and Dppa3. Interestingly, eRNAs produced at the -45 enhancer specifically regulate Dppa3 expression by stabilizing looping of the -45 enhancer and Dppa3. Our work illustrates that genomic editing is required to determine enhancer function and points to a method to selectively target a subset of super-enhancer-regulated genes by depleting eRNAs. Twit Text FOAVip Super-Enhancers at the Nanog Locus Differentially Regulate Neighboring Pluripotency-Associated Genes ????Cell Rep http://www.kidney.de/pget.php?&tw=1&pmid=27681417 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27681417 #FOAvip English Wikipedia <ref>{{Cite pmid|27681417 }}</ref> Super-Enhancers at the Nanog Locus Differentially Regulate Neighboring Pluripotency-Associated Genes #MMPMID27681417 Blinka S ; Reimer MH Jr ; Pulakanti K ; Rao S Cell Rep 2016[Sep]; 17 (1 ): 19-28 PMID27681417 show ga Super-enhancers are tissue-specific cis-regulatory elements that drive expression of genes associated with cell identity and malignancy. A cardinal feature of super-enhancers is that they are transcribed to produce enhancer-derived RNAs (eRNAs). It remains unclear whether super-enhancers robustly activate genes in situ and whether their functions are attributable to eRNAs or the DNA element. CRISPR/Cas9 was used to systematically delete three discrete super-enhancers at the Nanog locus in embryonic stem cells, revealing functional differences in Nanog transcriptional regulation. One distal super-enhancer 45 kb upstream of Nanog (-45 enhancer) regulates both nearest neighbor genes, Nanog and Dppa3. Interestingly, eRNAs produced at the -45 enhancer specifically regulate Dppa3 expression by stabilizing looping of the -45 enhancer and Dppa3. Our work illustrates that genomic editing is required to determine enhancer function and points to a method to selectively target a subset of super-enhancer-regulated genes by depleting eRNAs. |*Enhancer Elements, Genetic [MESH] |Animals [MESH] |CRISPR-Cas Systems [MESH] |Chromosomal Proteins, Non-Histone [MESH] |Clustered Regularly Interspaced Short Palindromic Repeats [MESH] |Endonucleases/genetics/metabolism [MESH] |Gene Editing [MESH] |Gene Expression Regulation [MESH] |Human Embryonic Stem Cells/cytology/*metabolism [MESH] |Humans [MESH] |Mice [MESH] |NIH 3T3 Cells [MESH] |Nanog Homeobox Protein/*genetics/metabolism [MESH] |Pluripotent Stem Cells/cytology/*metabolism [MESH] |Primary Cell Culture [MESH] |Proteins/*genetics/metabolism [MESH] |RNA, Long Noncoding/*genetics/metabolism [MESH]Super-Enhancers at the Nanog Locus Differentially Regulate Neighboring(epmc).pdf DeepDyve Pubget Overpricing