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10.1016/bs.mie.2014.10.042

http://scihub22266oqcxt.onion/10.1016/bs.mie.2014.10.042
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C5110928!5110928!25707270
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suck abstract from ncbi


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pmid25707270      Methods+Enzymol 2015 ; 552 (ä): 3-22
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  • Measuring synchrony in the mammalian central circadian circuit #MMPMID25707270
  • Herzog ED; Kiss IZ; Mazuski C
  • Methods Enzymol 2015[]; 552 (ä): 3-22 PMID25707270show ga
  • Circadian clocks control daily rhythms in physiology and behavior across all phyla. These rhythms are intrinsic to individual cells that must synchronize to their environment and to each other to anticipate daily events. Recent advances in recording from large numbers of cells for many circadian cycles have enabled researchers to begin to evaluate the mechanisms and consequences of intercellular circadian synchrony. Consequently, methods have been adapted to estimate the period, phase and amplitude of individual circadian cells and calculate synchrony between cells. Stable synchronization requires that the cells share a common period. As a result, synchronized cells maintain constant phase relationships to each (e.g. with cell 1 peaking an hour before cell 2 each cycle). This chapter reviews how circadian rhythms are recorded from single mammalian cells and details methods for measuring their period and phase synchrony. These methods have been useful, for example, in showing that specific neuropeptides are essential to maintain synchrony among circadian cells.
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