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2016 ; 39
(3
): 316-328
Nephropedia Template TP
Cayuso J
; Dzementsei A
; Fischer JC
; Karemore G
; Caviglia S
; Bartholdson J
; Wright GJ
; Ober EA
Dev Cell
2016[Nov]; 39
(3
): 316-328
PMID27825440
show ga
Positioning organs in the body often requires the movement of multiple tissues,
yet the molecular and cellular mechanisms coordinating such movements are largely
unknown. Here, we show that bidirectional signaling between EphrinB1 and EphB3b
coordinates the movements of the hepatic endoderm and adjacent lateral plate
mesoderm (LPM), resulting in asymmetric positioning of the zebrafish liver.
EphrinB1 in hepatoblasts regulates directional migration and mediates
interactions with the LPM, where EphB3b controls polarity and movement of the
LPM. EphB3b in the LPM concomitantly repels hepatoblasts to move leftward into
the liver bud. Cellular protrusions controlled by Eph/Ephrin signaling mediate
hepatoblast motility and long-distance cell-cell contacts with the LPM beyond
immediate tissue interfaces. Mechanistically, intracellular EphrinB1 domains
mediate EphB3b-independent hepatoblast extension formation, while EpB3b
interactions cause their destabilization. We propose that bidirectional short-
and long-distance cell interactions between epithelial and mesenchyme-like
tissues coordinate liver bud formation and laterality via cell repulsion.