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Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Infect+Dis+Obstet+Gynecol 2016 ; 2016 (ä): ä Nephropedia Template TP
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Development of a Novel Test for Simultaneous Bacterial Identification and Antibiotic Susceptibility #MMPMID27872553
Faro J; Mitchell M; Chen YJ; Kamal S; Riddle G; Faro S
Infect Dis Obstet Gynecol 2016[]; 2016 (ä): ä PMID27872553show ga
Background. Elucidation of a pathogen's antimicrobial susceptibility requires subculture after the organism is first isolated. This takes several days, requiring patients to be treated with broad-spectrum antibiotics. This approach contributes to the development of bacterial resistance. Methods. Microtiter wells were coated with a polyclonal antibody targeting the pathogen of interest. Bacterial suspensions were added in the presence/absence of selected antibiotics. After washing, captured bacteria were detected. Findings. Group B streptococcus (GBS), Enterococcus faecalis, and Neisseria gonorrhoeae were each detected at 105?bacteria/mL following a 20-minute incubation period. Susceptibility to select antibiotics was discernable following a 6-hour incubation period (GBS and Enterococcus). Sensitivity was increased to 10?2?bacteria/mL for GBS, 10?1?bacteria/mL for E. faecalis, and 101?bacteria/mL for N. gonorrhoeae following 18?24-hour culture. Conclusion. This novel assay allows for the highly sensitive and specific identification of a pathogen and simultaneous determination of its antimicrobial susceptibility in a reduced time.