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10.1155/2016/5293034

http://scihub22266oqcxt.onion/10.1155/2016/5293034
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C5107225!5107225!27872553
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suck abstract from ncbi


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pmid27872553      Infect+Dis+Obstet+Gynecol 2016 ; 2016 (ä): ä
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  • Development of a Novel Test for Simultaneous Bacterial Identification and Antibiotic Susceptibility #MMPMID27872553
  • Faro J; Mitchell M; Chen YJ; Kamal S; Riddle G; Faro S
  • Infect Dis Obstet Gynecol 2016[]; 2016 (ä): ä PMID27872553show ga
  • Background. Elucidation of a pathogen's antimicrobial susceptibility requires subculture after the organism is first isolated. This takes several days, requiring patients to be treated with broad-spectrum antibiotics. This approach contributes to the development of bacterial resistance. Methods. Microtiter wells were coated with a polyclonal antibody targeting the pathogen of interest. Bacterial suspensions were added in the presence/absence of selected antibiotics. After washing, captured bacteria were detected. Findings. Group B streptococcus (GBS), Enterococcus faecalis, and Neisseria gonorrhoeae were each detected at 105?bacteria/mL following a 20-minute incubation period. Susceptibility to select antibiotics was discernable following a 6-hour incubation period (GBS and Enterococcus). Sensitivity was increased to 10?2?bacteria/mL for GBS, 10?1?bacteria/mL for E. faecalis, and 101?bacteria/mL for N. gonorrhoeae following 18?24-hour culture. Conclusion. This novel assay allows for the highly sensitive and specific identification of a pathogen and simultaneous determination of its antimicrobial susceptibility in a reduced time.
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