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2016 ; 2016
(ä): 5293034
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Development of a Novel Test for Simultaneous Bacterial Identification and
Antibiotic Susceptibility
#MMPMID27872553
Faro J
; Mitchell M
; Chen YJ
; Kamal S
; Riddle G
; Faro S
Infect Dis Obstet Gynecol
2016[]; 2016
(ä): 5293034
PMID27872553
show ga
Background. Elucidation of a pathogen's antimicrobial susceptibility requires
subculture after the organism is first isolated. This takes several days,
requiring patients to be treated with broad-spectrum antibiotics. This approach
contributes to the development of bacterial resistance. Methods. Microtiter wells
were coated with a polyclonal antibody targeting the pathogen of interest.
Bacterial suspensions were added in the presence/absence of selected antibiotics.
After washing, captured bacteria were detected. Findings. Group B streptococcus
(GBS), Enterococcus faecalis, and Neisseria gonorrhoeae were each detected at
10(5)?bacteria/mL following a 20-minute incubation period. Susceptibility to
select antibiotics was discernable following a 6-hour incubation period (GBS and
Enterococcus). Sensitivity was increased to 10(-2)?bacteria/mL for GBS,
10(-1)?bacteria/mL for E. faecalis, and 10(1)?bacteria/mL for N. gonorrhoeae
following 18-24-hour culture. Conclusion. This novel assay allows for the highly
sensitive and specific identification of a pathogen and simultaneous
determination of its antimicrobial susceptibility in a reduced time.