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10.1074/jbc.M116.728774 http://scihub22266oqcxt.onion/10.1074/jbc.M116.728774 C5104915!5104915!27645993     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Biol+Chem 2016 ; 291 (46): 23906-14 Nephropedia Template TP {{tp|p=27645993|t=2016. Circadian Oscillations of NADH Redox State Using a Heterologous Metabolic Sensor in Mammalian Cells* |pdf=|usr=}}{{27645993}} gab.com Text Circadian Oscillations of NADH Redox State Using a Heterologous Metabolic Sensor in Mammalian Cells* JO: J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=27645993 #FOAvip It is known that there are mechanistic links between circadian clocks and metabolic cycles. Reduced nicotinamide adenine dinucleotide (NADH) is a key metabolic cofactor in all living cells; however, it is not known whether levels of NADH oscillate or not. Here we employed REX, a bacterial NADH-binding protein, fused to the VP16 activator to convert intracellular endogenous redox balance into transcriptional readouts by a reporter gene in mammalian cells. EMSA results show that the DNA binding activity of both T- and S-REX::VP16 fusions is decreased with a reduced-to-oxidized cofactor ratio increase. Transient and stabilized cell lines bearing the REX::VP16 and the REX binding operator (ROP) exhibit two circadian luminescence cycles. Consistent with these results, NADH oscillations are observed in host cells, indicating REX can act as a NADH sensor to report intracellular dynamic redox homeostasis in mammalian cells in real time. NADH oscillations provide another metabolic signal for coupling the circadian clock and cellular metabolic states. Twit Text FOAVip Circadian Oscillations of NADH Redox State Using a Heterologous Metabolic Sensor in Mammalian Cells* ????J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=27645993 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27645993 #FOAvip English Wikipedia <ref>{{Cite pmid|27645993}}</ref> Circadian Oscillations of NADH Redox State Using a Heterologous Metabolic Sensor in Mammalian Cells* #MMPMID27645993 Huang G; Zhang Y; Shan Y; Yang S; Chelliah Y; Wang H; Takahashi JS J Biol Chem 2016[Nov]; 291 (46): 23906-14 PMID27645993show ga It is known that there are mechanistic links between circadian clocks and metabolic cycles. Reduced nicotinamide adenine dinucleotide (NADH) is a key metabolic cofactor in all living cells; however, it is not known whether levels of NADH oscillate or not. Here we employed REX, a bacterial NADH-binding protein, fused to the VP16 activator to convert intracellular endogenous redox balance into transcriptional readouts by a reporter gene in mammalian cells. EMSA results show that the DNA binding activity of both T- and S-REX::VP16 fusions is decreased with a reduced-to-oxidized cofactor ratio increase. Transient and stabilized cell lines bearing the REX::VP16 and the REX binding operator (ROP) exhibit two circadian luminescence cycles. Consistent with these results, NADH oscillations are observed in host cells, indicating REX can act as a NADH sensor to report intracellular dynamic redox homeostasis in mammalian cells in real time. NADH oscillations provide another metabolic signal for coupling the circadian clock and cellular metabolic states. äCircadian Oscillations of NADH Redox State Using a Heterologous Metabo(epmc).pdf DeepDyve Pubget Overpricing