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2016 ; 579
(ä): 255-76
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Tools for Model Building and Optimization into Near-Atomic Resolution Electron
Cryo-Microscopy Density Maps
#MMPMID27572730
DiMaio F
; Chiu W
Methods Enzymol
2016[]; 579
(ä): 255-76
PMID27572730
show ga
Electron cryo-microscopy (cryoEM) has advanced dramatically to become a viable
tool for high-resolution structural biology research. The ultimate outcome of a
cryoEM study is an atomic model of a macromolecule or its complex with
interacting partners. This chapter describes a variety of algorithms and software
to build a de novo model based on the cryoEM 3D density map, to optimize the
model with the best stereochemistry restraints and finally to validate the model
with proper protocols. The full process of atomic structure determination from a
cryoEM map is described. The tools outlined in this chapter should prove
extremely valuable in revealing atomic interactions guided by cryoEM data.