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10.1364/BOE.7.003958 http://scihub22266oqcxt.onion/10.1364/BOE.7.003958 C5102538!5102538!27867707     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biomed+Opt+Express 2016 ; 7 (10): 3958-67 Nephropedia Template TP {{tp|p=27867707|t=2016. Scanless functional imaging of hippocampal networks using patterned two-photon illumination through GRIN lenses |pdf=|usr=}}{{27867707}} gab.com Text Scanless functional imaging of hippocampal networks using patterned two-photon illumination through GRIN lenses JO: Biomed Opt Express http://www.kidney.de/pget.php?&tw=1&pmid=27867707 #FOAvip Patterned illumination through the phase modulation of light is increasingly recognized as a powerful tool to investigate biological tissues in combination with two-photon excitation and light-sensitive molecules. However, to date two-photon patterned illumination has only been coupled to traditional microscope objectives, thus limiting the applicability of these methods to superficial biological structures. Here, we show that phase modulation can be used to efficiently project complex two-photon light patterns, including arrays of points and large shapes, in the focal plane of graded index (GRIN) lenses. Moreover, using this approach in combination with the genetically encoded calcium indicator GCaMP6, we validate our system performing scanless functional imaging in rodent hippocampal networks in vivo ~1.2 mm below the brain surface. Our results open the way to the application of patterned illumination approaches to deep regions of highly scattering biological tissues, such as the mammalian brain. Twit Text FOAVip Scanless functional imaging of hippocampal networks using patterned two-photon illumination through GRIN lenses ????Biomed Opt Express http://www.kidney.de/pget.php?&tw=1&pmid=27867707 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27867707 #FOAvip English Wikipedia <ref>{{Cite pmid|27867707}}</ref> Scanless functional imaging of hippocampal networks using patterned two-photon illumination through GRIN lenses #MMPMID27867707 Moretti C; Antonini A; Bovetti S; Liberale C; Fellin T Biomed Opt Express 2016[Oct]; 7 (10): 3958-67 PMID27867707show ga Patterned illumination through the phase modulation of light is increasingly recognized as a powerful tool to investigate biological tissues in combination with two-photon excitation and light-sensitive molecules. However, to date two-photon patterned illumination has only been coupled to traditional microscope objectives, thus limiting the applicability of these methods to superficial biological structures. Here, we show that phase modulation can be used to efficiently project complex two-photon light patterns, including arrays of points and large shapes, in the focal plane of graded index (GRIN) lenses. Moreover, using this approach in combination with the genetically encoded calcium indicator GCaMP6, we validate our system performing scanless functional imaging in rodent hippocampal networks in vivo ~1.2 mm below the brain surface. Our results open the way to the application of patterned illumination approaches to deep regions of highly scattering biological tissues, such as the mammalian brain. äScanless functional imaging of hippocampal networks using patterned tw(epmc).pdf DeepDyve Pubget Overpricing