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2016 ; 14
(5
): 4650-4658
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Analysis of altered microRNA expression profiles in the kidney tissues of
ethylene glycol-induced hyperoxaluric rats
#MMPMID27748900
Liu Z
; Jiang H
; Yang J
; Wang T
; Ding Y
; Liu J
; Wang S
; Ye Z
Mol Med Rep
2016[Nov]; 14
(5
): 4650-4658
PMID27748900
show ga
Calcium oxalate stones account for >80% of urinary stones, however the mechanisms
underlying their formation remains to be elucidated. Hyperoxaluria serves an
important role in the pathophysiological process of stone formation. In the
present study, differences in the miRNA expression profiles between experimental
hyperoxaluric rats and normal rats were analyzed, in order to identify target
genes and signaling pathways involved in the pathogenesis of hyperoxaluria.
Ethylene glycol and ammonium chloride was fed to male hyperoxaluric rats (EXP)
and normal age?matched male rats (CON). The oxalate concentration in the urine of
each experimental rat was collected every 24 h and measured on day 14. Three rats
exhibiting the highest concentrations were selected for microarray analysis.
Microarray analysis was performed to evaluate differences in the expression of
microRNA (miRNA) in the kidney tissues from EXP and CON groups, and miRNAs that
exhibited a >2?fold or a <0.5?fold alteration in expression between these groups
were screened for differential expression patterns according to the threshold
P?values. Reverse transcription?quantitative polymerase chain reaction analysis
was employed to confirm the microarray results. In order to predict the potential
role of miRNAs in pathophysiological processes, gene ontology (GO), pathway and
target prediction analyses were conducted. A total of 28 miRNAs were observed to
be differentially expressed (>2?fold change) between EXP and CON groups. Among
these miRNAs, 20 were upregulated and 8 were downregulated. GO and pathway
analyses revealed that the insulin resistance and
phosphatidylinositol?bisphosphonate 3?kinase/AKT serine threonine kinase
signaling pathways were potentially associated with miRNA regulation in this
setting. In conclusion, the results of the present study identified
differentially expressed miRNAs in hyperoxaluric rats, and provided a novel
perspective for the role of miRNAs in the formation of calcium oxalate stones.
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