Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1002/acn3.326 http://scihub22266oqcxt.onion/10.1002/acn3.326 C5099529!5099529!27844029     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Ann+Clin+Transl+Neurol 2016 ; 3 (11): 828-43 Nephropedia Template TP {{tp|p=27844029|t=2016. Dose?dependent inhibition of demyelination and microglia activation by IVIG |pdf=|usr=}}{{27844029}} gab.com Text Dose dependent inhibition of demyelination and microglia activation by IVIG JO: Ann Clin Transl Neurol http://www.kidney.de/pget.php?&tw=1&pmid=27844029 #FOAvip Objective: Intravenous immunoglobulin (IVIG) is an established treatment for numerous autoimmune conditions. Clinical trials of IVIG for multiple sclerosis, using diverse dose regimens, yielded controversial results. The aim of this study is to dissect IVIG effector mechanisms on demyelination in an ex vivo model of the central nervous system (CNS)?immune interface. Methods: Using organotypic cerebellar slice cultures (OSC) from transgenic mice expressing green fluorescent protein (GFP) in oligodendrocytes/myelin, we induced extensive immune?mediated demyelination and oligodendrocyte loss with an antibody specific for myelin oligodendrocyte glycoprotein (MOG) and complement. Protective IVIG effects were assessed by live imaging of GFP expression, confocal microscopy, immunohistochemistry, gene expression analysis and flow cytometry. Results: IVIG protected OSC from demyelination in a dose?dependent manner, which was at least partly attributed to interference with complement?mediated oligodendroglia damage, while binding of the anti?MOG antibody was not prevented. Staining with anti?CD68 antibodies and flow cytometry confirmed that IVIG prevented microglia activation and oligodendrocyte death, respectively. Equimolar IVIG?derived Fab fragments or monoclonal IgG did not protect OSC, while Fc fragments derived from a polyclonal mixture of human IgG were at least as potent as intact IVIG. Interpretation: Both intact IVIG and Fc fragments exert a dose?dependent protective effect on antibody?mediated CNS demyelination and microglia activation by interfering with the complement cascade and, presumably, interacting with local immune cells. Although this experimental model lacks blood?brain barrier and peripheral immune components, our findings warrant further studies on optimal dose finding and alternative modes of application to enhance local IVIG concentrations at the site of tissue damage. Twit Text FOAVip Dose dependent inhibition of demyelination and microglia activation by IVIG ????Ann Clin Transl Neurol http://www.kidney.de/pget.php?&tw=1&pmid=27844029 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27844029 #FOAvip English Wikipedia <ref>{{Cite pmid|27844029}}</ref> Dose?dependent inhibition of demyelination and microglia activation by IVIG #MMPMID27844029 Winter M; Baksmeier C; Steckel J; Barman S; Malviya M; Harrer?Kuster M; Hartung H; Goebels N Ann Clin Transl Neurol 2016[Nov]; 3 (11): 828-43 PMID27844029show ga Objective: Intravenous immunoglobulin (IVIG) is an established treatment for numerous autoimmune conditions. Clinical trials of IVIG for multiple sclerosis, using diverse dose regimens, yielded controversial results. The aim of this study is to dissect IVIG effector mechanisms on demyelination in an ex vivo model of the central nervous system (CNS)?immune interface. Methods: Using organotypic cerebellar slice cultures (OSC) from transgenic mice expressing green fluorescent protein (GFP) in oligodendrocytes/myelin, we induced extensive immune?mediated demyelination and oligodendrocyte loss with an antibody specific for myelin oligodendrocyte glycoprotein (MOG) and complement. Protective IVIG effects were assessed by live imaging of GFP expression, confocal microscopy, immunohistochemistry, gene expression analysis and flow cytometry. Results: IVIG protected OSC from demyelination in a dose?dependent manner, which was at least partly attributed to interference with complement?mediated oligodendroglia damage, while binding of the anti?MOG antibody was not prevented. Staining with anti?CD68 antibodies and flow cytometry confirmed that IVIG prevented microglia activation and oligodendrocyte death, respectively. Equimolar IVIG?derived Fab fragments or monoclonal IgG did not protect OSC, while Fc fragments derived from a polyclonal mixture of human IgG were at least as potent as intact IVIG. Interpretation: Both intact IVIG and Fc fragments exert a dose?dependent protective effect on antibody?mediated CNS demyelination and microglia activation by interfering with the complement cascade and, presumably, interacting with local immune cells. Although this experimental model lacks blood?brain barrier and peripheral immune components, our findings warrant further studies on optimal dose finding and alternative modes of application to enhance local IVIG concentrations at the site of tissue damage. äDose?dependent inhibition of demyelination and microglia activation by(epmc).pdf DeepDyve Pubget Overpricing