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2016 ; 2016
(ä): 1936386
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Regulation of Autophagy-Related Protein and Cell Differentiation by High Mobility
Group Box 1 Protein in Adipocytes
#MMPMID27843198
Feng H
; Yu L
; Zhang G
; Liu G
; Yang C
; Wang H
; Song X
Mediators Inflamm
2016[]; 2016
(ä): 1936386
PMID27843198
show ga
High mobility group box 1 protein (HMGB1) is a molecule related to the
development of inflammation. Autophagy is vital to maintain cellular homeostasis
and protect against inflammation of adipocyte injury. Our recent work focused on
the relationship of HMGB1 and autophagy in 3T3-L1 cells. In vivo experimental
results showed that, compared with the normal-diet group, the high-fat diet mice
displayed an increase in adipocyte size in the epididymal adipose tissues. The
expression levels of HMGB1 and LC3II also increased in epididymal adipose tissues
in high-fat diet group compared to the normal-diet mice. The in vitro results
indicated that HMGB1 protein treatment increased LC3II formation in 3T3-L1
preadipocytes in contrast to that in the control group. Furthermore, LC3II
formation was inhibited through HMGB1 knockdown by siRNA. Treatment with the
HMGB1 protein enhanced LC3II expression after 2 and 4 days but decreased the
expression after 8 and 10 days among various differentiation stages of
adipocytes. By contrast, FABP4 expression decreased on the fourth day and
increased on the eighth day. Hence, the HMGB1 protein modulated autophagy-related
proteins and lipid-metabolism-related genes in adipocytes and could be a new
target for treatment of obesity and related metabolic diseases.