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10.1038/ncomms13287 http://scihub22266oqcxt.onion/10.1038/ncomms13287 C5097163!5097163 !27811843     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27811843 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Commun 2016 ; 7 (ä): 13287 Nephropedia Template TP {{tp|p=27811843 |t=2016. Long non-coding RNA GAS5 controls human embryonic stem cell self-renewal by maintaining NODAL signalling |pdf=|usr=}}{{27811843 }} gab.com Text Long non-coding RNA GAS5 controls human embryonic stem cell self-renewal by maintaining NODAL signalling JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=27811843 #FOAvip Long non-coding RNAs (lncRNAs) are known players in the regulatory circuitry of the self-renewal in human embryonic stem cells (hESCs). However, most hESC-specific lncRNAs remain uncharacterized. Here we demonstrate that growth-arrest-specific transcript 5 (GAS5), a known tumour suppressor and growth arrest-related lncRNA, is highly expressed and directly regulated by pluripotency factors OCT4 and SOX2 in hESCs. Phenotypic analysis shows that GAS5 knockdown significantly impairs hESC self-renewal, but its overexpression significantly promotes hESC self-renewal. Using RNA sequencing and functional analysis, we demonstrate that GAS5 maintains NODAL signalling by protecting NODAL expression from miRNA-mediated degradation. Therefore, we propose that the above pluripotency factors, GAS5 and NODAL form a feed-forward signalling loop that maintains hESC self-renewal. As this regulatory function of GAS5 is stem cell specific, our findings also indicate that the functions of lncRNAs may vary in different cell types due to competing endogenous mechanisms. Twit Text FOAVip Long non-coding RNA GAS5 controls human embryonic stem cell self-renewal by maintaining NODAL signalling ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=27811843 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27811843 #FOAvip English Wikipedia <ref>{{Cite pmid|27811843 }}</ref> Long non-coding RNA GAS5 controls human embryonic stem cell self-renewal by maintaining NODAL signalling #MMPMID27811843 Xu C ; Zhang Y ; Wang Q ; Xu Z ; Jiang J ; Gao Y ; Gao M ; Kang J ; Wu M ; Xiong J ; Ji K ; Yuan W ; Wang Y ; Liu H Nat Commun 2016[Nov]; 7 (ä): 13287 PMID27811843 show ga Long non-coding RNAs (lncRNAs) are known players in the regulatory circuitry of the self-renewal in human embryonic stem cells (hESCs). However, most hESC-specific lncRNAs remain uncharacterized. Here we demonstrate that growth-arrest-specific transcript 5 (GAS5), a known tumour suppressor and growth arrest-related lncRNA, is highly expressed and directly regulated by pluripotency factors OCT4 and SOX2 in hESCs. Phenotypic analysis shows that GAS5 knockdown significantly impairs hESC self-renewal, but its overexpression significantly promotes hESC self-renewal. Using RNA sequencing and functional analysis, we demonstrate that GAS5 maintains NODAL signalling by protecting NODAL expression from miRNA-mediated degradation. Therefore, we propose that the above pluripotency factors, GAS5 and NODAL form a feed-forward signalling loop that maintains hESC self-renewal. As this regulatory function of GAS5 is stem cell specific, our findings also indicate that the functions of lncRNAs may vary in different cell types due to competing endogenous mechanisms. |Cell Line [MESH] |Cell Self Renewal/*genetics [MESH] |Gene Knockdown Techniques [MESH] |Human Embryonic Stem Cells/*physiology [MESH] |Humans [MESH] |MicroRNAs/metabolism [MESH] |Nodal Protein/*genetics/metabolism [MESH] |Octamer Transcription Factor-3/metabolism [MESH] |RNA, Long Noncoding/*physiology [MESH] |SOXB1 Transcription Factors/metabolism [MESH] |Sequence Analysis, RNA [MESH]Long non-coding RNA GAS5 controls human embryonic stem cell self-renew(epmc).pdf DeepDyve Pubget Overpricing