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10.1038/ncomms13330 http://scihub22266oqcxt.onion/10.1038/ncomms13330 C5097136!5097136!27804970     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2016 ; 7 (ä): ä Nephropedia Template TP {{tp|p=27804970|t=2016. Engineering and optimising deaminase fusions for genome editing |pdf=|usr=}}{{27804970}} gab.com Text Engineering and optimising deaminase fusions for genome editing JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=27804970 #FOAvip Precise editing is essential for biomedical research and gene therapy. Yet, homology-directed genome modification is limited by the requirements for genomic lesions, homology donors and the endogenous DNA repair machinery. Here we engineered programmable cytidine deaminases and test if we could introduce site-specific cytidine to thymidine transitions in the absence of targeted genomic lesions. Our programmable deaminases effectively convert specific cytidines to thymidines with 13% efficiency in Escherichia coli and 2.5% in human cells. However, off-target deaminations were detected more than 150?bp away from the target site. Moreover, whole genome sequencing revealed that edited bacterial cells did not harbour chromosomal abnormalities but demonstrated elevated global cytidine deamination at deaminase intrinsic binding sites. Therefore programmable deaminases represent a promising genome editing tool in prokaryotes and eukaryotes. Future engineering is required to overcome the processivity and the intrinsic DNA binding affinity of deaminases for safer therapeutic applications. Twit Text FOAVip Engineering and optimising deaminase fusions for genome editing ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=27804970 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27804970 #FOAvip English Wikipedia <ref>{{Cite pmid|27804970}}</ref> Engineering and optimising deaminase fusions for genome editing #MMPMID27804970 Yang L; Briggs AW; Chew WL; Mali P; Guell M; Aach J; Goodman DB; Cox D; Kan Y; Lesha E; Soundararajan V; Zhang F; Church G Nat Commun 2016[]; 7 (ä): ä PMID27804970show ga Precise editing is essential for biomedical research and gene therapy. Yet, homology-directed genome modification is limited by the requirements for genomic lesions, homology donors and the endogenous DNA repair machinery. Here we engineered programmable cytidine deaminases and test if we could introduce site-specific cytidine to thymidine transitions in the absence of targeted genomic lesions. Our programmable deaminases effectively convert specific cytidines to thymidines with 13% efficiency in Escherichia coli and 2.5% in human cells. However, off-target deaminations were detected more than 150?bp away from the target site. Moreover, whole genome sequencing revealed that edited bacterial cells did not harbour chromosomal abnormalities but demonstrated elevated global cytidine deamination at deaminase intrinsic binding sites. Therefore programmable deaminases represent a promising genome editing tool in prokaryotes and eukaryotes. Future engineering is required to overcome the processivity and the intrinsic DNA binding affinity of deaminases for safer therapeutic applications. äEngineering and optimising deaminase fusions for genome editing (epmc).pdf DeepDyve Pubget Overpricing