Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1007/978-1-4939-3378-5_12 http://scihub22266oqcxt.onion/10.1007/978-1-4939-3378-5_12 C5094191!5094191!26721489     free     free     free       Methods+Mol+Biol 2016 ; 1402 (ä): 147-64 Nephropedia Template TP {{tp|p=26721489|t=2016. Visualizing Long Noncoding RNAs on Chromatin |pdf=|usr=}}{{26721489}} gab.com Text Visualizing Long Noncoding RNAs on Chromatin JO: Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=26721489 #FOAvip Fluorescence in situ hybridization (FISH) enables the detection of specific nucleic acid sequences within single cells. For example, RNA FISH provides information on both the expression level and localization of RNA transcripts and, when combined with detection of associated proteins and chromatin modifications, can lend essential insights into long noncoding RNA (lncRNA) function. Epigenetic effects have been postulated for many lncRNAs, but shown for only a few. Advances in in situ techniques and microscopy, however, now allow for visualization of lncRNAs that are expressed at very low levels or are not very stable. FISH-based detections of RNA and DNA coupled with immunological staining of proteins/histone modifications offer the possibility to connect lncRNAs to epigenetic effects. Here, we describe an integrated set of protocols to detect, individually or in combination, specific RNAs, DNAs, proteins, and histone modifications in single cells at a high level of sensitivity using conventional fluorescence microscopy. Twit Text FOAVip Visualizing Long Noncoding RNAs on Chromatin ????Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=26721489 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26721489 #FOAvip English Wikipedia <ref>{{Cite pmid|26721489}}</ref> Visualizing Long Noncoding RNAs on Chromatin #MMPMID26721489 Hinten M; Maclary E; Gayen S; Harris C; Kalantry S Methods Mol Biol 2016[]; 1402 (ä): 147-64 PMID26721489show ga Fluorescence in situ hybridization (FISH) enables the detection of specific nucleic acid sequences within single cells. For example, RNA FISH provides information on both the expression level and localization of RNA transcripts and, when combined with detection of associated proteins and chromatin modifications, can lend essential insights into long noncoding RNA (lncRNA) function. Epigenetic effects have been postulated for many lncRNAs, but shown for only a few. Advances in in situ techniques and microscopy, however, now allow for visualization of lncRNAs that are expressed at very low levels or are not very stable. FISH-based detections of RNA and DNA coupled with immunological staining of proteins/histone modifications offer the possibility to connect lncRNAs to epigenetic effects. Here, we describe an integrated set of protocols to detect, individually or in combination, specific RNAs, DNAs, proteins, and histone modifications in single cells at a high level of sensitivity using conventional fluorescence microscopy. äVisualizing Long Noncoding RNAs on Chromatin (epmc).pdf DeepDyve Pubget Overpricing