Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27802845
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 J+Extracell+Vesicles
2016 ; 5
(ä): 32945
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Techniques used for the isolation and characterization of extracellular vesicles:
results of a worldwide survey
#MMPMID27802845
Gardiner C
; Di Vizio D
; Sahoo S
; Théry C
; Witwer KW
; Wauben M
; Hill AF
J Extracell Vesicles
2016[]; 5
(ä): 32945
PMID27802845
show ga
Extracellular vesicles (EVs) represent an important mode of intercellular
communication. Research in this field has grown rapidly in the last few years,
and there is a plethora of techniques for the isolation and characterization of
EVs, many of which are poorly standardized. EVs are heterogeneous in size, origin
and molecular constituents, with considerable overlap in size and phenotype
between different populations of EVs. Little is known about current practices for
the isolation, purification and characterization of EVs. We report here the first
large, detailed survey of current worldwide practices for the isolation and
characterization of EVs. Conditioned cell culture media was the most widely used
material (83%). Ultracentrifugation remains the most commonly used isolation
method (81%) with 59% of respondents use a combination of methods. Only 9% of
respondents used only 1 characterization method, with others using 2 or more
methods. Sample volume, sample type and downstream application all influenced the
isolation and characterization techniques employed.