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10.1002/cbf.3193 http://scihub22266oqcxt.onion/10.1002/cbf.3193 C5089641!5089641 !27273381     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27273381 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Cell+Biochem+Funct 2016 ; 34 (5 ): 326-33 Nephropedia Template TP {{tp|p=27273381 |t=2016. Suppressive effect of microRNA-29b on hepatic stellate cell activation and its crosstalk with TGF-?1/Smad3 |pdf=|usr=}}{{27273381 }} gab.com Text Suppressive effect of microRNA-29b on hepatic stellate cell activation and its crosstalk with TGF- 1/Smad3 JO: Cell Biochem Funct http://www.kidney.de/pget.php?&tw=1&pmid=27273381 #FOAvip The microRNA (miR)-29 family is closely associated with fibrotic processes by virtue of its low expression in many tissues during organ fibrosis. The present study investigated whether miR-29b overexpression suppressed hepatic stellate cell (HSC) activation and its interactions with transforming growth factor (TGF)-?1/mothers against decapentaplegic homolog 3 (Smad3), a classical signal transduction pathway contributing to the activation of HSCs. The results showed that transfection of LX-2 (human HSC) cells with miR-29b mimic or pSUPER-Smad3 silencing (si)RNA resulted in significantly increased expression of miR-29b and decreased expression of Smad3. miR-29b overexpression inhibited proliferation of LX-2 cells 24?h after transfection. Both miR-29b overexpression and Smad3 silencing antagonized the effects of TGF-?1 on the expression of ?-smooth muscle actin (?-SMA) and collagen type I (col-1). Furthermore, infection with miR-29b mimics suppressed Smad3 and TGF-?1 expression, suggesting that miR-29b inhibited LX-2 activation mediated by both Smad3 and TGF-?1. Nevertheless, primary miR-29a/b1, miR-29b2/c and mature miR-29b were downregulated by TGF-?1 and stimulated by Smad3 silencing, suggesting that TGF-?1/Smad3 signalling pathway regulate not just mature miR-29b but also its transcription. In summary, our results show overwhelming evidence corroborating the suppressive effect of miR-29b on TGF-?1-induced LX-2 cell activation. The results also revealed the existence of crosstalk between miR-29b and TGF-?1/Smad3 during LX-2 activation, suggesting a feedback loop between miR-29b and TGF-?1/Smad3 signalling that promotes liver fibrosis. Copyright © 2016 The Authors. Cell Biochemistry and Function published by John Wiley & Sons, Ltd. Twit Text FOAVip Suppressive effect of microRNA-29b on hepatic stellate cell activation and its crosstalk with TGF- 1/Smad3 ????Cell Biochem Funct http://www.kidney.de/pget.php?&tw=1&pmid=27273381 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27273381 #FOAvip English Wikipedia <ref>{{Cite pmid|27273381 }}</ref> Suppressive effect of microRNA-29b on hepatic stellate cell activation and its crosstalk with TGF-?1/Smad3 #MMPMID27273381 Liang C ; Bu S ; Fan X Cell Biochem Funct 2016[Jul]; 34 (5 ): 326-33 PMID27273381 show ga The microRNA (miR)-29 family is closely associated with fibrotic processes by virtue of its low expression in many tissues during organ fibrosis. The present study investigated whether miR-29b overexpression suppressed hepatic stellate cell (HSC) activation and its interactions with transforming growth factor (TGF)-?1/mothers against decapentaplegic homolog 3 (Smad3), a classical signal transduction pathway contributing to the activation of HSCs. The results showed that transfection of LX-2 (human HSC) cells with miR-29b mimic or pSUPER-Smad3 silencing (si)RNA resulted in significantly increased expression of miR-29b and decreased expression of Smad3. miR-29b overexpression inhibited proliferation of LX-2 cells 24?h after transfection. Both miR-29b overexpression and Smad3 silencing antagonized the effects of TGF-?1 on the expression of ?-smooth muscle actin (?-SMA) and collagen type I (col-1). Furthermore, infection with miR-29b mimics suppressed Smad3 and TGF-?1 expression, suggesting that miR-29b inhibited LX-2 activation mediated by both Smad3 and TGF-?1. Nevertheless, primary miR-29a/b1, miR-29b2/c and mature miR-29b were downregulated by TGF-?1 and stimulated by Smad3 silencing, suggesting that TGF-?1/Smad3 signalling pathway regulate not just mature miR-29b but also its transcription. In summary, our results show overwhelming evidence corroborating the suppressive effect of miR-29b on TGF-?1-induced LX-2 cell activation. The results also revealed the existence of crosstalk between miR-29b and TGF-?1/Smad3 during LX-2 activation, suggesting a feedback loop between miR-29b and TGF-?1/Smad3 signalling that promotes liver fibrosis. Copyright © 2016 The Authors. Cell Biochemistry and Function published by John Wiley & Sons, Ltd. |Actins/metabolism [MESH] |Cell Proliferation [MESH] |Cells, Cultured [MESH] |Collagen Type I/metabolism [MESH] |Gene Expression Regulation [MESH] |Hepatic Stellate Cells/*cytology/*metabolism [MESH] |Humans [MESH] |MicroRNAs/genetics/*metabolism [MESH] |Signal Transduction [MESH] |Smad3 Protein/*metabolism [MESH] |Transfection [MESH]Suppressive effect of microRNA-29b on hepatic stellate cell activation(epmc).pdf DeepDyve Pubget Overpricing