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10.1042/BCJ20160422 http://scihub22266oqcxt.onion/10.1042/BCJ20160422 C5085863!5085863!27623775     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biochem+J 2016 ; 473 (21): 3819-36 Nephropedia Template TP {{tp|p=27623775|t=2016. Quaternary structures of opsin in live cells revealed by FRET spectrometry |pdf=|usr=}}{{27623775}} gab.com Text Quaternary structures of opsin in live cells revealed by FRET spectrometry JO: Biochem J http://www.kidney.de/pget.php?&tw=1&pmid=27623775 #FOAvip Rhodopsin is a prototypical G-protein coupled receptor (GPCR) that initiates phototransduction in the retina. The receptor consists of the apoprotein opsin covalently linked to the inverse agonist 11-cis retinal. Rhodopsin and opsin have been shown to form oligomers within the outer segment disc membranes of rod photoreceptor cells. However, the physiological relevance of the observed oligomers has been questioned since observations were made on samples prepared from the retina at low temperatures. To investigate the oligomeric status of opsin in live cells at body temperatures, we utilized a novel approach called FRET spectrometry, which previously has allowed the determination of the stoichiometry and geometry (i.e., quaternary structure) of various GPCRs. In the current study, we have extended the method to additionally determine whether or not a mixture of oligomeric forms of opsin exists and in what proportion. Application of this improved method revealed that opsin expressed in live Chinese hamster ovary (CHO) cells at 37 °C exists as oligomers of various sizes. At lower concentrations, opsin existed in an equilibrium of dimers and tetramers. The tetramers were in the shape of a near-rhombus. At higher concentrations of the receptor, higher order oligomers began to form. Thus, a mixture of different oligomeric forms of opsin is present in the membrane of live CHO cells and oligomerization occurs in a concentration-dependent manner. The general principles underlying the concentration-dependent oligomerization of opsin may be universal and apply to other GPCRs as well. Twit Text FOAVip Quaternary structures of opsin in live cells revealed by FRET spectrometry ????Biochem J http://www.kidney.de/pget.php?&tw=1&pmid=27623775 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27623775 #FOAvip English Wikipedia <ref>{{Cite pmid|27623775}}</ref> Quaternary structures of opsin in live cells revealed by FRET spectrometry #MMPMID27623775 Mishra AK; Gragg M; Stoneman M; Biener G; Oliver JA; Miszta P; Filipek S; Raicu V; Park PSH Biochem J 2016[Nov]; 473 (21): 3819-36 PMID27623775show ga Rhodopsin is a prototypical G-protein coupled receptor (GPCR) that initiates phototransduction in the retina. The receptor consists of the apoprotein opsin covalently linked to the inverse agonist 11-cis retinal. Rhodopsin and opsin have been shown to form oligomers within the outer segment disc membranes of rod photoreceptor cells. However, the physiological relevance of the observed oligomers has been questioned since observations were made on samples prepared from the retina at low temperatures. To investigate the oligomeric status of opsin in live cells at body temperatures, we utilized a novel approach called FRET spectrometry, which previously has allowed the determination of the stoichiometry and geometry (i.e., quaternary structure) of various GPCRs. In the current study, we have extended the method to additionally determine whether or not a mixture of oligomeric forms of opsin exists and in what proportion. Application of this improved method revealed that opsin expressed in live Chinese hamster ovary (CHO) cells at 37 °C exists as oligomers of various sizes. At lower concentrations, opsin existed in an equilibrium of dimers and tetramers. The tetramers were in the shape of a near-rhombus. At higher concentrations of the receptor, higher order oligomers began to form. Thus, a mixture of different oligomeric forms of opsin is present in the membrane of live CHO cells and oligomerization occurs in a concentration-dependent manner. The general principles underlying the concentration-dependent oligomerization of opsin may be universal and apply to other GPCRs as well. äQuaternary structures of opsin in live cells revealed by FRET spectrom(epmc).pdf DeepDyve Pubget Overpricing