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Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Int+J+Mol+Sci 2016 ; 17 (10): ä Nephropedia Template TP
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Diatom Valve Three-Dimensional Representation: A New Imaging Method Based on Combined Microscopies #MMPMID27690008
Ferrara MA; De Tommasi E; Coppola G; De Stefano L; Rea I; Dardano P
Int J Mol Sci 2016[Oct]; 17 (10): ä PMID27690008show ga
The frustule of diatoms, unicellular microalgae, shows very interesting photonic features, generally related to its complicated and quasi-periodic micro- and nano-structure. In order to simulate light propagation inside and through this natural structure, it is important to develop three-dimensional (3D) models for synthetic replica with high spatial resolution. In this paper, we present a new method that generates images of microscopic diatoms with high definition, by merging scanning electron microscopy and digital holography microscopy or atomic force microscopy data. Starting from two digital images, both acquired separately with standard characterization procedures, a high spatial resolution (?z = ?/20, ?x = ?y ? 100 nm, at least) 3D model of the object has been generated. Then, the two sets of data have been processed by matrix formalism, using an original mathematical algorithm implemented on a commercially available software. The developed methodology could be also of broad interest in the design and fabrication of micro-opto-electro-mechanical systems.