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10.1007/s12195-016-0457-4 http://scihub22266oqcxt.onion/10.1007/s12195-016-0457-4 C5084850!5084850!27800035     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Mol+Bioeng 2016 ; 9 (3): 315-24 Nephropedia Template TP {{tp|p=27800035|t=2016. Oncogene Knockdown via Active Loading of Small RNAs into Extracellular Vesicles by Sonication |pdf=|usr=}}{{27800035}} gab.com Text Oncogene Knockdown via Active Loading of Small RNAs into Extracellular Vesicles by Sonication JO: Cell Mol Bioeng http://www.kidney.de/pget.php?&tw=1&pmid=27800035 #FOAvip Extracellular vesicles (EVs), including exosomes and microvesicles, have emerged as promising drug delivery vehicles for small RNAs (siRNA and miRNA) due to their natural role in intercellular RNA transport. However, the application of EVs for therapeutic RNA delivery may be limited by loading approaches that can induce cargo aggregation or degradation. Here, we report the use of sonication as a means to actively load functional small RNAs into EVs. Conditions under which EVs could be loaded with small RNAs with minimal detectable aggregation were identified, and EVs loaded with therapeutic siRNA via sonication were observed to be taken up by recipient cells and capable of target mRNA knockdown leading to reduced protein expression. This system was ultimately applied to reduce expression of HER2, an oncogenic receptor tyrosine kinase that critically mediates breast cancer development and progression, and could be extended to other therapeutic targets. These results define important parameters informing the application of sonication as a small RNA loading method for EVs and demonstrate the potential utility of this approach for versatile cancer therapy. Twit Text FOAVip Oncogene Knockdown via Active Loading of Small RNAs into Extracellular Vesicles by Sonication ????Cell Mol Bioeng http://www.kidney.de/pget.php?&tw=1&pmid=27800035 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27800035 #FOAvip English Wikipedia <ref>{{Cite pmid|27800035}}</ref> Oncogene Knockdown via Active Loading of Small RNAs into Extracellular Vesicles by Sonication #MMPMID27800035 Lamichhane TN; Jeyaram A; Patel DB; Parajuli B; Livingston NK; Arumugasaamy N; Schardt JS; Jay SM Cell Mol Bioeng 2016[Sep]; 9 (3): 315-24 PMID27800035show ga Extracellular vesicles (EVs), including exosomes and microvesicles, have emerged as promising drug delivery vehicles for small RNAs (siRNA and miRNA) due to their natural role in intercellular RNA transport. However, the application of EVs for therapeutic RNA delivery may be limited by loading approaches that can induce cargo aggregation or degradation. Here, we report the use of sonication as a means to actively load functional small RNAs into EVs. Conditions under which EVs could be loaded with small RNAs with minimal detectable aggregation were identified, and EVs loaded with therapeutic siRNA via sonication were observed to be taken up by recipient cells and capable of target mRNA knockdown leading to reduced protein expression. This system was ultimately applied to reduce expression of HER2, an oncogenic receptor tyrosine kinase that critically mediates breast cancer development and progression, and could be extended to other therapeutic targets. These results define important parameters informing the application of sonication as a small RNA loading method for EVs and demonstrate the potential utility of this approach for versatile cancer therapy. äOncogene Knockdown via Active Loading of Small RNAs into Extracellular(epmc).pdf DeepDyve Pubget Overpricing