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10.1371/journal.pone.0165047 http://scihub22266oqcxt.onion/10.1371/journal.pone.0165047 C5077102!5077102 !27776169     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27776169 &cmd=llinks): Failed to open stream: HTTP request failed! 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Hepatitis C virus Broadly Neutralizing Monoclonal Antibodies Isolated 25 Years after Spontaneous Clearance |pdf=|usr=}}{{27776169 }} gab.com Text Hepatitis C virus Broadly Neutralizing Monoclonal Antibodies Isolated 25 Years after Spontaneous Clearance JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=27776169 #FOAvip Hepatitis C virus (HCV) is world-wide a major cause of liver related morbidity and mortality. No vaccine is available to prevent HCV infection. To design an effective vaccine, understanding immunity against HCV is necessary. The memory B cell repertoire was characterized from an intravenous drug user who spontaneously cleared HCV infection 25 years ago. CD27+IgG+ memory B cells were immortalized using BCL6 and Bcl-xL. These immortalized B cells were used to study antibody-mediated immunity against the HCV E1E2 glycoproteins. Five E1E2 broadly reactive antibodies were isolated: 3 antibodies showed potent neutralization of genotype 1 to 4 using HCV pseudotyped particles, whereas the other 2 antibodies neutralized genotype 1, 2 and 3 or 1 and 2 only. All antibodies recognized non-linear epitopes on E2. Finally, except for antibody AT12-011, which recognized an epitope consisting of antigenic domain C /AR2 and AR5, all other four antibodies recognized epitope II and domain B. These data show that a subject, who spontaneously cleared HCV infection 25 years ago, still has circulating memory B cells that are able to secrete broadly neutralizing antibodies. Presence of such memory B cells strengthens the argument for undertaking the development of an HCV vaccine. Twit Text FOAVip Hepatitis C virus Broadly Neutralizing Monoclonal Antibodies Isolated 25 Years after Spontaneous Clearance ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=27776169 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27776169 #FOAvip English Wikipedia <ref>{{Cite pmid|27776169 }}</ref> Hepatitis C virus Broadly Neutralizing Monoclonal Antibodies Isolated 25 Years after Spontaneous Clearance #MMPMID27776169 Merat SJ ; Molenkamp R ; Wagner K ; Koekkoek SM ; van de Berg D ; Yasuda E ; Böhne M ; Claassen YB ; Grady BP ; Prins M ; Bakker AQ ; de Jong MD ; Spits H ; Schinkel J ; Beaumont T PLoS One 2016[]; 11 (10 ): e0165047 PMID27776169 show ga Hepatitis C virus (HCV) is world-wide a major cause of liver related morbidity and mortality. No vaccine is available to prevent HCV infection. To design an effective vaccine, understanding immunity against HCV is necessary. The memory B cell repertoire was characterized from an intravenous drug user who spontaneously cleared HCV infection 25 years ago. CD27+IgG+ memory B cells were immortalized using BCL6 and Bcl-xL. These immortalized B cells were used to study antibody-mediated immunity against the HCV E1E2 glycoproteins. Five E1E2 broadly reactive antibodies were isolated: 3 antibodies showed potent neutralization of genotype 1 to 4 using HCV pseudotyped particles, whereas the other 2 antibodies neutralized genotype 1, 2 and 3 or 1 and 2 only. All antibodies recognized non-linear epitopes on E2. Finally, except for antibody AT12-011, which recognized an epitope consisting of antigenic domain C /AR2 and AR5, all other four antibodies recognized epitope II and domain B. These data show that a subject, who spontaneously cleared HCV infection 25 years ago, still has circulating memory B cells that are able to secrete broadly neutralizing antibodies. Presence of such memory B cells strengthens the argument for undertaking the development of an HCV vaccine. |Adult [MESH] |Antibodies, Monoclonal/*isolation & purification [MESH] |Antibodies, Neutralizing/*isolation & purification [MESH] |B-Lymphocytes/cytology/immunology [MESH] |Epitopes/immunology [MESH] |Genotype [MESH] |Hepacivirus/genetics/*immunology/metabolism [MESH] |Hepatitis C Antibodies/*isolation & purification [MESH] |Hepatitis C/*blood/therapy [MESH] |Humans [MESH] |Male [MESH] |Substance Abuse, Intravenous/virology [MESH] |Viral Envelope Proteins/*immunology [MESH]Hepatitis C virus Broadly Neutralizing Monoclonal Antibodies Isolated (epmc).pdf DeepDyve Pubget Overpricing