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10.1002/anie.201509310 http://scihub22266oqcxt.onion/10.1002/anie.201509310 C5074284!5074284!27119221     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Angew+Chem+Int+Ed+Engl 2016 ; 55 (20): 5896-903 Nephropedia Template TP {{tp|p=27119221|t=2016. From Chemical Mutagenesis to Post?Expression Mutagenesis: A 50 Year Odyssey |pdf=|usr=}}{{27119221}} gab.com Text From Chemical Mutagenesis to Post Expression Mutagenesis: A 50 Year Odyssey JO: Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=27119221 #FOAvip Site?directed (gene) mutagenesis has been the most useful method available for the conversion of one amino acid residue of a given protein into another. Until relatively recently, this strategy was limited to the twenty standard amino acids. The ongoing maturation of stop codon suppression and related technologies for unnatural amino acid incorporation has greatly expanded access to nonstandard amino acids by expanding the scope of the translational apparatus. However, the necessity for translation of genetic changes restricts the diversity of residues that may be incorporated. Herein we highlight an alternative approach, termed post?expression mutagenesis, which operates at the level of the very functional biomolecules themselves. Using the lens of retrosynthesis, we highlight prospects for new strategies in protein modification, alteration, and construction which will enable protein science to move beyond the constraints of the ?translational filter? and lead to a true synthetic biology. Twit Text FOAVip From Chemical Mutagenesis to Post Expression Mutagenesis: A 50 Year Odyssey ????Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=27119221 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27119221 #FOAvip English Wikipedia <ref>{{Cite pmid|27119221}}</ref> From Chemical Mutagenesis to Post?Expression Mutagenesis: A 50 Year Odyssey #MMPMID27119221 Wright TH; Vallée MRJ; Davis BG Angew Chem Int Ed Engl 2016[May]; 55 (20): 5896-903 PMID27119221show ga Site?directed (gene) mutagenesis has been the most useful method available for the conversion of one amino acid residue of a given protein into another. Until relatively recently, this strategy was limited to the twenty standard amino acids. The ongoing maturation of stop codon suppression and related technologies for unnatural amino acid incorporation has greatly expanded access to nonstandard amino acids by expanding the scope of the translational apparatus. However, the necessity for translation of genetic changes restricts the diversity of residues that may be incorporated. Herein we highlight an alternative approach, termed post?expression mutagenesis, which operates at the level of the very functional biomolecules themselves. Using the lens of retrosynthesis, we highlight prospects for new strategies in protein modification, alteration, and construction which will enable protein science to move beyond the constraints of the ?translational filter? and lead to a true synthetic biology. äFrom Chemical Mutagenesis to Post?Expression Mutagenesis: A 50 Year Od(epmc).pdf DeepDyve Pubget Overpricing