Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1111/tan.12775 http://scihub22266oqcxt.onion/10.1111/tan.12775 C5071754!5071754!27060279     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       HLA 2016 ; 87 (5): 356-66 Nephropedia Template TP {{tp|p=27060279|t=2016. Many de novo donor?specific antibodies recognize ?2?microglobulin?free, but not intact HLA heterodimers |pdf=|usr=}}{{27060279}} gab.com Text Many de novo donor specific antibodies recognize 2 microglobulin free, but not intact HLA heterodimers JO: HLA http://www.kidney.de/pget.php?&tw=1&pmid=27060279 #FOAvip Solid?phase single antigen bead (SAB) assays are standard of care for detection and identification of donor?specific antibody (DSA) in patients who receive solid organ transplantation (SOT). While several studies have documented the reproducibility and sensitivity of SAB testing for DSA, there are little data available concerning its specificity. This study describes the identification of antibodies to ?2?microglobulin?free human leukocyte antigen (?2?m?fHLA) heavy chains on SAB arrays and provides a reassessment of the clinical relevance of DSA testing by this platform. Post?transplant sera from 55 patients who were positive for de novo donor?specific antibodies on a SAB solid?phase immunoassay were tested under denaturing conditions in order to identify antibodies reactive with ?2?m?fHLA or native HLA (nHLA). Antibodies to ?2?m?fHLA were present in nearly half of patients being monitored in the post?transplant period. The frequency of antibodies to ?2?m?fHLA was similar among DSA and HLA antigens that were irrelevant to the transplant (non?DSA). Among the seven patients with clinical or pathologic antibody?mediated rejection (AMR), none had antibodies to ?2?m?fHLA exclusively; thus, the clinical relevance of ?2?m?fHLA is unclear. Our data suggests that SAB testing produces false positive reactions due to the presence of ?2?m?fHLA and these can lead to inappropriate assignment of unacceptable antigens during transplant listing and possibly inaccurate identification of DSA in the post?transplant period. Twit Text FOAVip Many de novo donor specific antibodies recognize 2 microglobulin free, but not intact HLA heterodimers ????HLA http://www.kidney.de/pget.php?&tw=1&pmid=27060279 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27060279 #FOAvip English Wikipedia <ref>{{Cite pmid|27060279}}</ref> Many de novo donor?specific antibodies recognize ?2?microglobulin?free, but not intact HLA heterodimers #MMPMID27060279 Michel K; Santella R; Steers J; Sahajpal A; Downey FX; Thohan V; Oaks M HLA 2016[May]; 87 (5): 356-66 PMID27060279show ga Solid?phase single antigen bead (SAB) assays are standard of care for detection and identification of donor?specific antibody (DSA) in patients who receive solid organ transplantation (SOT). While several studies have documented the reproducibility and sensitivity of SAB testing for DSA, there are little data available concerning its specificity. This study describes the identification of antibodies to ?2?microglobulin?free human leukocyte antigen (?2?m?fHLA) heavy chains on SAB arrays and provides a reassessment of the clinical relevance of DSA testing by this platform. Post?transplant sera from 55 patients who were positive for de novo donor?specific antibodies on a SAB solid?phase immunoassay were tested under denaturing conditions in order to identify antibodies reactive with ?2?m?fHLA or native HLA (nHLA). Antibodies to ?2?m?fHLA were present in nearly half of patients being monitored in the post?transplant period. The frequency of antibodies to ?2?m?fHLA was similar among DSA and HLA antigens that were irrelevant to the transplant (non?DSA). Among the seven patients with clinical or pathologic antibody?mediated rejection (AMR), none had antibodies to ?2?m?fHLA exclusively; thus, the clinical relevance of ?2?m?fHLA is unclear. Our data suggests that SAB testing produces false positive reactions due to the presence of ?2?m?fHLA and these can lead to inappropriate assignment of unacceptable antigens during transplant listing and possibly inaccurate identification of DSA in the post?transplant period. äMany de novo donor?specific antibodies recognize ?2?microglobulin?free(epmc).pdf DeepDyve Pubget Overpricing