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10.1038/nchembio.2172 http://scihub22266oqcxt.onion/10.1038/nchembio.2172 C5069139!5069139!27618187     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Chem+Biol 2016 ; 12 (11): 937-43 Nephropedia Template TP {{tp|p=27618187|t=2016. Lactate Metabolism is Associated with Mammalian Mitochondria |pdf=|usr=}}{{27618187}} gab.com Text Lactate Metabolism is Associated with Mammalian Mitochondria JO: Nat Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=27618187 #FOAvip It is well established that lactate secreted by fermenting cells can be oxidized or used as a gluconeogenic substrate by other cells and tissues. Within the fermenting cell itself, however, it is generally assumed that lactate is produced to replenish NAD+ and then is secreted. Here we explored the possibility that cytosolic lactate is metabolized by the mitochondria of fermenting mammalian cells. We found that fermenting HeLa and H460 cells utilize exogenous lactate carbon to synthesize a large percentage of their lipids. With high-resolution mass spectrometry, we found that both 13C and 2-2H labels from enriched lactate enter the mitochondria. The lactate dehydrogenase (LDH) inhibitor oxamate decreased respiration of isolated mitochondria incubated in lactate, but not isolated mitochondria incubated in pyruvate. Additionally, transmission electron microscopy (TEM) showed that LDHB localizes to the mitochondria. Taken together, our results demonstrate a link between lactate metabolism and the mitochondria of fermenting mammalian cells. Twit Text FOAVip Lactate Metabolism is Associated with Mammalian Mitochondria ????Nat Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=27618187 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27618187 #FOAvip English Wikipedia <ref>{{Cite pmid|27618187}}</ref> Lactate Metabolism is Associated with Mammalian Mitochondria #MMPMID27618187 Chen YJ; Mahieu NG; Huang X; Singh M; Crawford PA; Johnson SL; Gross RW; Schaefer J; Patti GJ Nat Chem Biol 2016[Nov]; 12 (11): 937-43 PMID27618187show ga It is well established that lactate secreted by fermenting cells can be oxidized or used as a gluconeogenic substrate by other cells and tissues. Within the fermenting cell itself, however, it is generally assumed that lactate is produced to replenish NAD+ and then is secreted. Here we explored the possibility that cytosolic lactate is metabolized by the mitochondria of fermenting mammalian cells. We found that fermenting HeLa and H460 cells utilize exogenous lactate carbon to synthesize a large percentage of their lipids. With high-resolution mass spectrometry, we found that both 13C and 2-2H labels from enriched lactate enter the mitochondria. The lactate dehydrogenase (LDH) inhibitor oxamate decreased respiration of isolated mitochondria incubated in lactate, but not isolated mitochondria incubated in pyruvate. Additionally, transmission electron microscopy (TEM) showed that LDHB localizes to the mitochondria. Taken together, our results demonstrate a link between lactate metabolism and the mitochondria of fermenting mammalian cells. äLactate Metabolism is Associated with Mammalian Mitochondria (epmc).pdf DeepDyve Pubget Overpricing