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10.3346/jkms.2016.31.11.1673

http://scihub22266oqcxt.onion/10.3346/jkms.2016.31.11.1673
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C5056196!5056196!27709842
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suck abstract from ncbi


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pmid27709842      J+Korean+Med+Sci 2016 ; 31 (11): 1673-83
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  • Molecular Strain Typing of Mycobacterium tuberculosis: a Review of Frequently Used Methods #MMPMID27709842
  • Ei PW; Aung WW; Lee JS; Choi GE; Chang CL
  • J Korean Med Sci 2016[Nov]; 31 (11): 1673-83 PMID27709842show ga
  • Tuberculosis, caused by the bacterium Mycobacterium tuberculosis, remains one of the most serious global health problems. Molecular typing of M. tuberculosis has been used for various epidemiologic purposes as well as for clinical management. Currently, many techniques are available to type M. tuberculosis. Choosing the most appropriate technique in accordance with the existing laboratory conditions and the specific features of the geographic region is important. Insertion sequence IS6110-based restriction fragment length polymorphism (RFLP) analysis is considered the gold standard for the molecular epidemiologic investigations of tuberculosis. However, other polymerase chain reaction-based methods such as spacer oligonucleotide typing (spoligotyping), which detects 43 spacer sequence-interspersing direct repeats (DRs) in the genomic DR region; mycobacterial interspersed repetitive units?variable number tandem repeats, (MIRU-VNTR), which determines the number and size of tandem repetitive DNA sequences; repetitive-sequence-based PCR (rep-PCR), which provides high-throughput genotypic fingerprinting of multiple Mycobacterium species; and the recently developed genome-based whole genome sequencing methods demonstrate similar discriminatory power and greater convenience. This review focuses on techniques frequently used for the molecular typing of M. tuberculosis and discusses their general aspects and applications.
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