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10.1364/OPTICA.3.000659 http://scihub22266oqcxt.onion/10.1364/OPTICA.3.000659 C5050005!5050005!27722186     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Optica 2016 ; 3 (6): 3-6 Nephropedia Template TP {{tp|p=27722186|t=2016. Enhanced DNA imaging using super-resolution microscopy and simultaneous single-molecule orientation measurements |pdf=|usr=}}{{27722186}} gab.com Text Enhanced DNA imaging using super-resolution microscopy and simultaneous single-molecule orientation measurements JO: Optica http://www.kidney.de/pget.php?&tw=1&pmid=27722186 #FOAvip Single-molecule orientation measurements provide unparalleled insight into a multitude of biological and polymeric systems. We report a simple, high-throughput technique for measuring the azimuthal orientation and rotational dynamics of single fluorescent molecules, which is compatible with localization microscopy. Our method involves modulating the polarization of an excitation laser, and analyzing the corresponding intensities emitted by single dye molecules and their modulation amplitudes. To demonstrate our approach, we use intercalating and groove-binding dyes to obtain super-resolved images of stretched DNA strands through binding-induced turn-on of fluorescence. By combining our image data with thousands of dye molecule orientation measurements, we develop a means of probing the structure of individual DNA strands, while also characterizing dye-DNA interactions. This approach may hold promise as a method for monitoring DNA conformation changes resulting from DNA-binding proteins. Twit Text FOAVip Enhanced DNA imaging using super-resolution microscopy and simultaneous single-molecule orientation measurements ????Optica http://www.kidney.de/pget.php?&tw=1&pmid=27722186 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27722186 #FOAvip English Wikipedia <ref>{{Cite pmid|27722186}}</ref> Enhanced DNA imaging using super-resolution microscopy and simultaneous single-molecule orientation measurements #MMPMID27722186 Backer AS; Lee MY; Moerner WE Optica 2016[]; 3 (6): 3-6 PMID27722186show ga Single-molecule orientation measurements provide unparalleled insight into a multitude of biological and polymeric systems. We report a simple, high-throughput technique for measuring the azimuthal orientation and rotational dynamics of single fluorescent molecules, which is compatible with localization microscopy. Our method involves modulating the polarization of an excitation laser, and analyzing the corresponding intensities emitted by single dye molecules and their modulation amplitudes. To demonstrate our approach, we use intercalating and groove-binding dyes to obtain super-resolved images of stretched DNA strands through binding-induced turn-on of fluorescence. By combining our image data with thousands of dye molecule orientation measurements, we develop a means of probing the structure of individual DNA strands, while also characterizing dye-DNA interactions. This approach may hold promise as a method for monitoring DNA conformation changes resulting from DNA-binding proteins. äEnhanced DNA imaging using super-resolution microscopy and simultaneou(epmc).pdf DeepDyve Pubget Overpricing