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10.1016/j.jid.2016.06.007 http://scihub22266oqcxt.onion/10.1016/j.jid.2016.06.007 C5042211!5042211 !27542298     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27542298 &cmd=llinks): Failed to open stream: HTTP request failed! 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Research Techniques Made Simple: The Application of CRISPR-Cas9 and Genome Editing in Investigative Dermatology |pdf=|usr=}}{{27542298 }} gab.com Text Research Techniques Made Simple: The Application of CRISPR-Cas9 and Genome Editing in Investigative Dermatology JO: J Invest Dermatol http://www.kidney.de/pget.php?&tw=1&pmid=27542298 #FOAvip Designer nucleases have gained widespread attention for their ability to precisely modify genomic DNA in a programmable manner. These genome-editing nucleases make double-stranded breaks at specified loci, and desired changes can be made to modify, ablate, or excise target genes. This technology has been used widely to develop human disease models in laboratory animals and to study gene functions by silencing, activating, or modifying them. Furthermore, the recent discovery of a bacterially derived programmable nuclease termed clustered regularly interspaced palindromic repeats (CRISPR)-associated protein 9 (Cas9) has revolutionized the field because of its versatility and wide applicability. In this article, we discuss various modalities used to achieve genome editing with an emphasis on CRISPR-Cas9. We discuss genome-editing strategies to either repair or ablate target genes, with emphasis on their applications for investigating dermatological diseases. Additionally, we highlight preclinical studies showing the potential of genome editing as a therapy for congenital blistering diseases and as an antimicrobial agent, and we discuss limitations and future directions of this technology. Twit Text FOAVip Research Techniques Made Simple: The Application of CRISPR-Cas9 and Genome Editing in Investigative Dermatology ????J Invest Dermatol http://www.kidney.de/pget.php?&tw=1&pmid=27542298 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27542298 #FOAvip English Wikipedia <ref>{{Cite pmid|27542298 }}</ref> Research Techniques Made Simple: The Application of CRISPR-Cas9 and Genome Editing in Investigative Dermatology #MMPMID27542298 Guitart JR Jr ; Johnson JL ; Chien WW J Invest Dermatol 2016[Sep]; 136 (9 ): e87-e93 PMID27542298 show ga Designer nucleases have gained widespread attention for their ability to precisely modify genomic DNA in a programmable manner. These genome-editing nucleases make double-stranded breaks at specified loci, and desired changes can be made to modify, ablate, or excise target genes. This technology has been used widely to develop human disease models in laboratory animals and to study gene functions by silencing, activating, or modifying them. Furthermore, the recent discovery of a bacterially derived programmable nuclease termed clustered regularly interspaced palindromic repeats (CRISPR)-associated protein 9 (Cas9) has revolutionized the field because of its versatility and wide applicability. In this article, we discuss various modalities used to achieve genome editing with an emphasis on CRISPR-Cas9. We discuss genome-editing strategies to either repair or ablate target genes, with emphasis on their applications for investigating dermatological diseases. Additionally, we highlight preclinical studies showing the potential of genome editing as a therapy for congenital blistering diseases and as an antimicrobial agent, and we discuss limitations and future directions of this technology. |Animals [MESH] |CRISPR-Cas Systems/*genetics [MESH] |Dermatology/*methods [MESH] |Forecasting [MESH] |Gene Editing/*methods [MESH] |Genetic Engineering/methods/*trends [MESH] |Humans [MESH]Research Techniques Made Simple: The Application of CRISPR-Cas9 and Ge(epmc).pdf DeepDyve Pubget Overpricing