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10.1021/jp4102916 http://scihub22266oqcxt.onion/10.1021/jp4102916 C5040483!5040483!24417324     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Phys+Chem+B 2014 ; 118 (12): 3281-90 Nephropedia Template TP {{tp|p=24417324|t=2014. On the potential of hyperpolarized water in biomolecular NMR studies |pdf=|usr=}}{{24417324}} gab.com Text On the potential of hyperpolarized water in biomolecular NMR studies JO: J Phys Chem B http://www.kidney.de/pget.php?&tw=1&pmid=24417324 #FOAvip A main obstacle arising when using ex-situ hyperpolarization to increase the sensitivity of biomolecular NMR, is the fast relaxation that macromolecular spins undergo upon being transferred from the polarizer to the spectrometer where their observation takes place. To cope with this limitation the present study explores the use of hyperpolarized water, as a means to enhance the sensitivity of nuclei in biomolecules. Methods to achieve proton polarizations in excess of 5% in water transferred into the NMR spectrometer were devised, as were methods enabling this polarization to last for up to 30 sec. Upon dissolving aminoacids and polypeptides sited at the spectrometer into such hyperpolarized water, a substantial enhancement of certain biomolecular amide and amine proton resonances was observed. This exchange driven 1H enhancement was further passed on to sidechain and to backbone nitrogens, owing to spontaneous one-bond Overhauser processes. 15N signal enhancements >500 over 11.7 T thermal counterparts could thus be imparted, in a kinetic process that enabled multi-scan signal averaging. Besides potential bioanalytical uses, this approach opens interesting possibilities in the monitoring of dynamic biomolecular processes -including solvent accessibility and exchange process. Twit Text FOAVip On the potential of hyperpolarized water in biomolecular NMR studies ????J Phys Chem B http://www.kidney.de/pget.php?&tw=1&pmid=24417324 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24417324 #FOAvip English Wikipedia <ref>{{Cite pmid|24417324}}</ref> On the potential of hyperpolarized water in biomolecular NMR studies #MMPMID24417324 Harris T; Szekely O; Frydman L J Phys Chem B 2014[Mar]; 118 (12): 3281-90 PMID24417324show ga A main obstacle arising when using ex-situ hyperpolarization to increase the sensitivity of biomolecular NMR, is the fast relaxation that macromolecular spins undergo upon being transferred from the polarizer to the spectrometer where their observation takes place. To cope with this limitation the present study explores the use of hyperpolarized water, as a means to enhance the sensitivity of nuclei in biomolecules. Methods to achieve proton polarizations in excess of 5% in water transferred into the NMR spectrometer were devised, as were methods enabling this polarization to last for up to 30 sec. Upon dissolving aminoacids and polypeptides sited at the spectrometer into such hyperpolarized water, a substantial enhancement of certain biomolecular amide and amine proton resonances was observed. This exchange driven 1H enhancement was further passed on to sidechain and to backbone nitrogens, owing to spontaneous one-bond Overhauser processes. 15N signal enhancements >500 over 11.7 T thermal counterparts could thus be imparted, in a kinetic process that enabled multi-scan signal averaging. Besides potential bioanalytical uses, this approach opens interesting possibilities in the monitoring of dynamic biomolecular processes -including solvent accessibility and exchange process. äOn the potential of hyperpolarized water in biomolecular NMR studies (epmc).pdf DeepDyve Pubget Overpricing