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10.1021/acs.jproteome.6b00316 http://scihub22266oqcxt.onion/10.1021/acs.jproteome.6b00316 C5036859!5036859!27193225     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Proteome+Res 2016 ; 15 (7): 2265-82 Nephropedia Template TP {{tp|p=27193225|t=2016. The Composition of Rosenthal Fibers, the Protein Aggregate Hallmark of Alexander Disease |pdf=|usr=}}{{27193225}} gab.com Text The Composition of Rosenthal Fibers, the Protein Aggregate Hallmark of Alexander Disease JO: J Proteome Res http://www.kidney.de/pget.php?&tw=1&pmid=27193225 #FOAvip Alexander disease (AxD) is a neurodegenerative disorder characterized by astrocytic protein aggregates called Rosenthal fibers (RFs). We used mouse models of AxD to determine the protein composition of RFs to obtain information about disease mechanisms, including the hypothesis that sequestration of proteins in RFs contributes to disease. A method was developed for RF enrichment, and analysis of the resulting fraction using iTRAQ mass spectrometry identified 77 proteins not previously associated with RFs. Three of five proteins selected for follow-up were confirmed enriched in the RF fraction by immunobloting of both the AxD mouse models and human patients: receptor for activated protein C kinase 1 (RACK1), G1/S-specific cyclin D2, and ATP-dependent RNA helicase DDX3X. Immunohistochemistry validated cyclin D2 as a new RF component, but results for RACK1 and DDX3X were equivocal. None of these was decreased in the non-RF fractions compared to controls. A similar result was obtained for the previously known RF component, alphaB-crystallin, which had been a candidate for sequestration. Thus no support was obtained for the sequestration hypothesis for AxD. Providing possible insight into disease progression, the association of several of the RF proteins with stress granules suggests a role for stress granules in the origin of RFs. Twit Text FOAVip The Composition of Rosenthal Fibers, the Protein Aggregate Hallmark of Alexander Disease ????J Proteome Res http://www.kidney.de/pget.php?&tw=1&pmid=27193225 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27193225 #FOAvip English Wikipedia <ref>{{Cite pmid|27193225}}</ref> The Composition of Rosenthal Fibers, the Protein Aggregate Hallmark of Alexander Disease #MMPMID27193225 Heaven MR; Flint D; Randall SM; Sosunov AA; Wilson L; Barnes S; Goldman JE; Muddiman DC; Brenner M J Proteome Res 2016[Jul]; 15 (7): 2265-82 PMID27193225show ga Alexander disease (AxD) is a neurodegenerative disorder characterized by astrocytic protein aggregates called Rosenthal fibers (RFs). We used mouse models of AxD to determine the protein composition of RFs to obtain information about disease mechanisms, including the hypothesis that sequestration of proteins in RFs contributes to disease. A method was developed for RF enrichment, and analysis of the resulting fraction using iTRAQ mass spectrometry identified 77 proteins not previously associated with RFs. Three of five proteins selected for follow-up were confirmed enriched in the RF fraction by immunobloting of both the AxD mouse models and human patients: receptor for activated protein C kinase 1 (RACK1), G1/S-specific cyclin D2, and ATP-dependent RNA helicase DDX3X. Immunohistochemistry validated cyclin D2 as a new RF component, but results for RACK1 and DDX3X were equivocal. None of these was decreased in the non-RF fractions compared to controls. A similar result was obtained for the previously known RF component, alphaB-crystallin, which had been a candidate for sequestration. Thus no support was obtained for the sequestration hypothesis for AxD. Providing possible insight into disease progression, the association of several of the RF proteins with stress granules suggests a role for stress granules in the origin of RFs. äThe Composition of Rosenthal Fibers, the Protein Aggregate Hallmark of(epmc).pdf DeepDyve Pubget Overpricing