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10.1073/pnas.1522478113 http://scihub22266oqcxt.onion/10.1073/pnas.1522478113 C5035885!5035885 !27601656     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27601656 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Proc+Natl+Acad+Sci+U+S+A 2016 ; 113 (38 ): 10571-6 Nephropedia Template TP {{tp|p=27601656 |t=2016. In B cells, phosphatidylinositol 5-phosphate 4-kinase-? synthesizes PI(4,5)P2 to impact mTORC2 and Akt signaling |pdf=|usr=}}{{27601656 }} gab.com Text In B cells, phosphatidylinositol 5-phosphate 4-kinase- synthesizes PI(4,5)P2 to impact mTORC2 and Akt signaling JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=27601656 #FOAvip Phosphatidylinositol 5-phosphate 4-kinases (PI5P4Ks) are enigmatic lipid kinases with physiological functions that are incompletely understood, not the least because genetic deletion and cell transfection have led to contradictory data. Here, we used the genetic tractability of DT40 cells to create cell lines in which endogenous PI5P4K? was removed, either stably by genetic deletion or transiently (within 1 h) by tagging the endogenous protein genomically with the auxin degron. In both cases, removal impacted Akt phosphorylation, and by leaving one PI5P4K? allele present but mutating it to be kinase-dead or have PI4P 5-kinase activity, we show that all of the effects on Akt phosphorylation were dependent on the ability of PI5P4K? to synthesize phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] rather than to remove PI5P. Although stable removal of PI5P4K? resulted in a pronounced decrease in Akt phosphorylation at Thr308 and Ser473, in part because of reduced plasma membrane PIP3, its acute removal led to an increase in Akt phosphorylation only at Ser473. This process invokes activation primarily of mammalian target of rapamycin complex 2 (mTORC2), which was confirmed by increased phosphorylation of other mTORC2 substrates. These findings establish PI5P4K? as a kinase that synthesizes a physiologically relevant pool of PI(4,5)P2 and as a regulator of mTORC2, and show a phenomenon similar to the "butterfly effect" described for phosphatidylinositol 3-kinase I? [Hart JR, et al. (2015) Proc Natl Acad Sci USA 112(4):1131-1136], whereby through apparently the same underlying mechanism, the removal of a protein's activity from a cell can have widely divergent effects depending on the time course of that removal. Twit Text FOAVip In B cells, phosphatidylinositol 5-phosphate 4-kinase- synthesizes PI(4,5)P2 to impact mTORC2 and Akt signaling ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=27601656 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27601656 #FOAvip English Wikipedia <ref>{{Cite pmid|27601656 }}</ref> In B cells, phosphatidylinositol 5-phosphate 4-kinase-? synthesizes PI(4,5)P2 to impact mTORC2 and Akt signaling #MMPMID27601656 Bulley SJ ; Droubi A ; Clarke JH ; Anderson KE ; Stephens LR ; Hawkins PT ; Irvine RF Proc Natl Acad Sci U S A 2016[Sep]; 113 (38 ): 10571-6 PMID27601656 show ga Phosphatidylinositol 5-phosphate 4-kinases (PI5P4Ks) are enigmatic lipid kinases with physiological functions that are incompletely understood, not the least because genetic deletion and cell transfection have led to contradictory data. Here, we used the genetic tractability of DT40 cells to create cell lines in which endogenous PI5P4K? was removed, either stably by genetic deletion or transiently (within 1 h) by tagging the endogenous protein genomically with the auxin degron. In both cases, removal impacted Akt phosphorylation, and by leaving one PI5P4K? allele present but mutating it to be kinase-dead or have PI4P 5-kinase activity, we show that all of the effects on Akt phosphorylation were dependent on the ability of PI5P4K? to synthesize phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] rather than to remove PI5P. Although stable removal of PI5P4K? resulted in a pronounced decrease in Akt phosphorylation at Thr308 and Ser473, in part because of reduced plasma membrane PIP3, its acute removal led to an increase in Akt phosphorylation only at Ser473. This process invokes activation primarily of mammalian target of rapamycin complex 2 (mTORC2), which was confirmed by increased phosphorylation of other mTORC2 substrates. These findings establish PI5P4K? as a kinase that synthesizes a physiologically relevant pool of PI(4,5)P2 and as a regulator of mTORC2, and show a phenomenon similar to the "butterfly effect" described for phosphatidylinositol 3-kinase I? [Hart JR, et al. (2015) Proc Natl Acad Sci USA 112(4):1131-1136], whereby through apparently the same underlying mechanism, the removal of a protein's activity from a cell can have widely divergent effects depending on the time course of that removal. |Animals [MESH] |B-Lymphocytes/enzymology [MESH] |Cell Line [MESH] |Chickens/genetics [MESH] |Humans [MESH] |Mechanistic Target of Rapamycin Complex 2/genetics/*metabolism [MESH] |Phosphatidylinositol Phosphates/*metabolism [MESH] |Phosphorylation/genetics [MESH] |Phosphotransferases (Alcohol Group Acceptor)/genetics/metabolism [MESH] |Phosphotransferases/*genetics/metabolism [MESH] |Proto-Oncogene Proteins c-akt/*genetics/metabolism [MESH] |Signal Transduction/genetics [MESH]In B cells, phosphatidylinositol 5-phosphate 4-kinase-? synthesizes PI(epmc).pdf DeepDyve Pubget Overpricing