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10.1186/s12935-016-0349-7 http://scihub22266oqcxt.onion/10.1186/s12935-016-0349-7 C5035454/?report=reader!5035454!27688736     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 284.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 284.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 284.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cancer+Cell+Int 2016 ; 16 (ä): ä Nephropedia Template TP {{tp|p=27688736|t=2016. Long noncoding RNA ANRIL is activated by hypoxia-inducible factor-1? and promotes osteosarcoma cell invasion and suppresses cell apoptosis upon hypoxia |pdf=|usr=}}{{27688736}} gab.com Text Long noncoding RNA ANRIL is activated by hypoxia-inducible factor-1 and promotes osteosarcoma cell invasion and suppresses cell apoptosis upon hypoxia JO: Cancer Cell Int http://www.kidney.de/pget.php?&tw=1&pmid=27688736 #FOAvip Background: Osteosarcoma is the most common malignancy of bone. Intratumoral hypoxia occurs in many solid tumors, where it is associated with the development of aggressive phenotype. ANRIL has been shown to be a long noncoding RNA that facilitates the progression of a number of malignancies. Yet, few studies have explored the expression pattern of ANRIL in osteosarcoma and the effect of hypoxia on ANRIL. Methods: We evaluated the expression levels of ANRIL in osteosarcoma tissues, adjacent normal tissues and cells with quantitative real-time polymerase chain reaction. Multiple approaches including luciferase reporter assay with nucleotide substitutions, chromatin immunoprecipitation assay and electrophoretic mobility shift assay were used to confirm the direct binding of HIF-1? to the ANRIL promoter region. SiRNA-based knockdown and other molecular biology techniques were employed to measure the effect of HIF-1? on the expression of ANRIL. Results: We found that the expression of ANRIL was upregulated in 15 pairs of osteosarcoma compared with adjacent normal tissues. We found that hypoxia is sufficient to upregulate ANRIL expression in osteosarcoma cells (MNNG and U2OS). HIF-1? directly binds to the putative hypoxia response element in the upstream region of ANRIL. What?s more, siRNA and small molecular inhibitors-mediated HIF-1? suppression attenuated ANRIL upregulation under hypoxic conditions. Upon hypoxia, ANRIL promoted cancer cell invasion and suppressed cell apoptosis. Conclusion: Taken together, these data suggest that HIF-1? may contribute to the upregulation of ANRIL in osteosarcoma under hypoxic conditions. ANRIL is involved in hypoxia-induced aggressive phenotype in osteosarcoma. Twit Text FOAVip Long noncoding RNA ANRIL is activated by hypoxia-inducible factor-1 and promotes osteosarcoma cell invasion and suppresses cell apoptosis upon hypoxia ????Cancer Cell Int http://www.kidney.de/pget.php?&tw=1&pmid=27688736 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27688736 #FOAvip English Wikipedia <ref>{{Cite pmid|27688736}}</ref> Long noncoding RNA ANRIL is activated by hypoxia-inducible factor-1? and promotes osteosarcoma cell invasion and suppresses cell apoptosis upon hypoxia #MMPMID27688736 Wei X; Wang C; Ma C; Sun W; Li H; Cai Z Cancer Cell Int 2016[]; 16 (ä): ä PMID27688736show ga Background: Osteosarcoma is the most common malignancy of bone. Intratumoral hypoxia occurs in many solid tumors, where it is associated with the development of aggressive phenotype. ANRIL has been shown to be a long noncoding RNA that facilitates the progression of a number of malignancies. Yet, few studies have explored the expression pattern of ANRIL in osteosarcoma and the effect of hypoxia on ANRIL. Methods: We evaluated the expression levels of ANRIL in osteosarcoma tissues, adjacent normal tissues and cells with quantitative real-time polymerase chain reaction. Multiple approaches including luciferase reporter assay with nucleotide substitutions, chromatin immunoprecipitation assay and electrophoretic mobility shift assay were used to confirm the direct binding of HIF-1? to the ANRIL promoter region. SiRNA-based knockdown and other molecular biology techniques were employed to measure the effect of HIF-1? on the expression of ANRIL. Results: We found that the expression of ANRIL was upregulated in 15 pairs of osteosarcoma compared with adjacent normal tissues. We found that hypoxia is sufficient to upregulate ANRIL expression in osteosarcoma cells (MNNG and U2OS). HIF-1? directly binds to the putative hypoxia response element in the upstream region of ANRIL. What?s more, siRNA and small molecular inhibitors-mediated HIF-1? suppression attenuated ANRIL upregulation under hypoxic conditions. Upon hypoxia, ANRIL promoted cancer cell invasion and suppressed cell apoptosis. Conclusion: Taken together, these data suggest that HIF-1? may contribute to the upregulation of ANRIL in osteosarcoma under hypoxic conditions. ANRIL is involved in hypoxia-induced aggressive phenotype in osteosarcoma. äLong noncoding RNA ANRIL is activated by hypoxia-inducible factor-1? a(epmc).pdf DeepDyve Pubget Overpricing