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10.1016/j.cell.2016.08.006 http://scihub22266oqcxt.onion/10.1016/j.cell.2016.08.006 C5034880!5034880!27594427     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell 2016 ; 166 (6): 1572-1584.e16 Nephropedia Template TP {{tp|p=27594427|t=2016. Polar positioning of phase-separated liquid compartments in cells regulated by an mRNA competition mechanism |pdf=|usr=}}{{27594427}} gab.com Text Polar positioning of phase-separated liquid compartments in cells regulated by an mRNA competition mechanism JO: Cell http://www.kidney.de/pget.php?&tw=1&pmid=27594427 #FOAvip P granules are non-membrane-bound RNA-protein compartments that are involved in germline development in C. elegans. They are liquids that condense at one end of the embryo by localized phase separation, driven by gradients of polarity proteins such as the mRNA-binding protein MEX-5. To probe how polarity proteins regulate phase separation, we combined biochemistry and theoretical modeling. We reconstitute P granule-like droplets in vitro using a single protein PGL-3. By combining in vitro reconstitution with measurements of intracellular concentrations, we show that competition between PGL-3 and MEX-5 for mRNA can regulate the formation of PGL-3 droplets. Using theory we show that in a MEX-5 gradient, this mRNA competition mechanism can drive a gradient of P granule assembly with similar spatial and temporal characteristics to P granule assembly in vivo. We conclude that gradients of polarity proteins can position RNP granules during development, by using RNA competition to regulate local phase separation. Twit Text FOAVip Polar positioning of phase-separated liquid compartments in cells regulated by an mRNA competition mechanism ????Cell http://www.kidney.de/pget.php?&tw=1&pmid=27594427 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27594427 #FOAvip English Wikipedia <ref>{{Cite pmid|27594427}}</ref> Polar positioning of phase-separated liquid compartments in cells regulated by an mRNA competition mechanism #MMPMID27594427 Saha S; Weber CA; Nousch M; Hoege C; Adame-Arana O; Hein MY; Osborne-Nishimura E; Mahamid J; Jahnel M; Jawerth L; Pozniakovski A; Eckmann CR; Jülicher F; Hyman AA Cell 2016[Sep]; 166 (6): 1572-1584.e16 PMID27594427show ga P granules are non-membrane-bound RNA-protein compartments that are involved in germline development in C. elegans. They are liquids that condense at one end of the embryo by localized phase separation, driven by gradients of polarity proteins such as the mRNA-binding protein MEX-5. To probe how polarity proteins regulate phase separation, we combined biochemistry and theoretical modeling. We reconstitute P granule-like droplets in vitro using a single protein PGL-3. By combining in vitro reconstitution with measurements of intracellular concentrations, we show that competition between PGL-3 and MEX-5 for mRNA can regulate the formation of PGL-3 droplets. Using theory we show that in a MEX-5 gradient, this mRNA competition mechanism can drive a gradient of P granule assembly with similar spatial and temporal characteristics to P granule assembly in vivo. We conclude that gradients of polarity proteins can position RNP granules during development, by using RNA competition to regulate local phase separation. äPolar positioning of phase-separated liquid compartments in cells regu(epmc).pdf DeepDyve Pubget Overpricing