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2016 ; 2016
(ä): 9125960
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Vibration Induces BAFF Overexpression and Aberrant O-Glycosylation of IgA1 in
Cultured Human Tonsillar Mononuclear Cells in IgA Nephropathy
#MMPMID27672662
Ye M
; Peng Y
; Liu C
; Yan W
; Peng X
; He L
; Liu H
; Liu F
Biomed Res Int
2016[]; 2016
(ä): 9125960
PMID27672662
show ga
Objective. To investigate the influence of in vitro vibratory stimulation of
human tonsillar mononuclear cells (TMCs). Methods. Fourteen IgA nephropathy
(IgAN) patients with chronic tonsillitis (CT) and 12 CT patients with no renal
pathology were enrolled. Group A TMCs were collected after 24 hours of culture
and used to determine baseline levels. TMCs in groups B, C, D, E, and F were
exposed to vibratory stimulation (60?Hz) for 0 (as the control group), 1, 3, 5,
and 10 minutes, respectively. Results. Baseline concentrations of
B-cell-activation factor (BAFF) and IgA1, BAFF mRNA expression, and aberrant
O-glycosylation IgA1 level were higher in the IgAN group as compared to that in
the CT group, and all increased after vibratory stimulation. Baseline mRNA
expressions of core ?1,3-galactosyltransferase (C1GALT1) and core
?1,3GalT-specific molecular chaperone (Cosmc) were lower in the IgAN group; the
levels decreased further after vibratory stimulation. Conclusion. In patients
with IgAN, vibratory stimulation of TMCs appears to induce IgA1 secretion through
activation of BAFF release and to aberrant O-glycosylation IgA1 by suppressing
C1GALT1 and Cosmc expression. In vitro vibratory stimulation of human TMCs mimics
the vibratory simulation of palatine tonsils produced by vocal cords during
phonation.