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10.1016/j.molcel.2016.08.011 http://scihub22266oqcxt.onion/10.1016/j.molcel.2016.08.011 C5031556!5031556!27618490     free     free     free       Mol+Cell 2016 ; 63 (6): 939-50 Nephropedia Template TP {{tp|p=27618490|t=2016. RNA Polymerase Pausing during Initial Transcription |pdf=|usr=}}{{27618490}} gab.com Text RNA Polymerase Pausing during Initial Transcription JO: Mol Cell http://www.kidney.de/pget.php?&tw=1&pmid=27618490 #FOAvip In bacteria, RNA polymerase (RNAP) initiates transcription by synthesizing short transcripts that are either released or extended to allow RNAP to escape from the promoter. The mechanism of initial transcription is unclear due to the presence of transient intermediates and molecular heterogeneity. Here, we studied initial transcription on a lac promoter using single-molecule fluorescence observations of DNA scrunching on immobilized transcription complexes. Our work revealed a long pause (?initiation pause,? ?20 s) after synthesis of a 6-mer RNA; such pauses can serve as regulatory checkpoints. Region sigma 3.2, which contains a loop blocking the RNA exit channel, was a major pausing determinant. We also obtained evidence for RNA backtracking during abortive initial transcription and for additional pausing prior to escape. We summarized our work in a model for initial transcription, in which pausing is controlled by a complex set of determinants that modulate the transition from a 6- to a 7-nt RNA. Twit Text FOAVip RNA Polymerase Pausing during Initial Transcription ????Mol Cell http://www.kidney.de/pget.php?&tw=1&pmid=27618490 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27618490 #FOAvip English Wikipedia <ref>{{Cite pmid|27618490}}</ref> RNA Polymerase Pausing during Initial Transcription #MMPMID27618490 Duchi D; Bauer D; Fernandez L; Evans G; Robb N; Hwang L; Gryte K; Tomescu A; Zawadzki P; Morichaud Z; Brodolin K; Kapanidis A Mol Cell 2016[Sep]; 63 (6): 939-50 PMID27618490show ga In bacteria, RNA polymerase (RNAP) initiates transcription by synthesizing short transcripts that are either released or extended to allow RNAP to escape from the promoter. The mechanism of initial transcription is unclear due to the presence of transient intermediates and molecular heterogeneity. Here, we studied initial transcription on a lac promoter using single-molecule fluorescence observations of DNA scrunching on immobilized transcription complexes. Our work revealed a long pause (?initiation pause,? ?20 s) after synthesis of a 6-mer RNA; such pauses can serve as regulatory checkpoints. Region sigma 3.2, which contains a loop blocking the RNA exit channel, was a major pausing determinant. We also obtained evidence for RNA backtracking during abortive initial transcription and for additional pausing prior to escape. We summarized our work in a model for initial transcription, in which pausing is controlled by a complex set of determinants that modulate the transition from a 6- to a 7-nt RNA. äRNA Polymerase Pausing during Initial Transcription (epmc).pdf DeepDyve Pubget Overpricing