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10.1016/j.biomaterials.2013.12.093 http://scihub22266oqcxt.onion/10.1016/j.biomaterials.2013.12.093 C5030820!5030820!24439414     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biomaterials 2014 ; 35 (10): 3252-62 Nephropedia Template TP {{tp|p=24439414|t=2014. Enhanced reseeding of decellularized rodent lungs with mouse embryonic stem cells |pdf=|usr=}}{{24439414}} gab.com Text Enhanced reseeding of decellularized rodent lungs with mouse embryonic stem cells JO: Biomaterials http://www.kidney.de/pget.php?&tw=1&pmid=24439414 #FOAvip Repopulation of decellularized lung scaffolds (DLS) is limited due to alterations in the repertoire and ratios of the residual extracellular matrix (ECM) proteins, characterized by e.g., the retention of type I collagen and loss of glycoproteins. We hypothesized that pre-treatment of decellularized matrices with defined ECM proteins, which match the repertoire of integrin receptors expressed by the cells to be seeded (e.g., embryonic stem cells) can increase the efficacy of the reseeding process. To test this hypothesis, we first determined the integrin receptors profile of mouse embryonic stem cells (mESCs). Mouse ESCs express ?3, ?5, ?6, ?9 and ?1, but not ?1, ?2 and ?4 integrin subunits, as established by Western blotting and adhesion to laminin and fibronectin, but not to collagens type I and IV. Reseeding of DLS with mESCs was inefficient (6.9 ± 0.5%), but was significantly enhanced (2.3 ± 0.1 fold) by pre-treating the scaffolds with media conditioned by A549 human lung adenocarcinoma cells, which we found to contain ~5 ?g/ml laminin. Furthermore, pre-treatment with A549-conditioned media resulted in a significantly more uniform distribution of the seeded mESCs throughout the engineered organ as compared to untreated DLS. Our study may advance whole lung engineering by stressing the importance of matching the integrin receptor repertoire of the seeded cells and the cell binding motifs of DLS. Twit Text FOAVip Enhanced reseeding of decellularized rodent lungs with mouse embryonic stem cells ????Biomaterials http://www.kidney.de/pget.php?&tw=1&pmid=24439414 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24439414 #FOAvip English Wikipedia <ref>{{Cite pmid|24439414}}</ref> Enhanced reseeding of decellularized rodent lungs with mouse embryonic stem cells #MMPMID24439414 Lecht S; Stabler CT; Rylander AL; Chiaverelli R; Schulman ES; Marcinkiewicz C; Lelkes PI Biomaterials 2014[Mar]; 35 (10): 3252-62 PMID24439414show ga Repopulation of decellularized lung scaffolds (DLS) is limited due to alterations in the repertoire and ratios of the residual extracellular matrix (ECM) proteins, characterized by e.g., the retention of type I collagen and loss of glycoproteins. We hypothesized that pre-treatment of decellularized matrices with defined ECM proteins, which match the repertoire of integrin receptors expressed by the cells to be seeded (e.g., embryonic stem cells) can increase the efficacy of the reseeding process. To test this hypothesis, we first determined the integrin receptors profile of mouse embryonic stem cells (mESCs). Mouse ESCs express ?3, ?5, ?6, ?9 and ?1, but not ?1, ?2 and ?4 integrin subunits, as established by Western blotting and adhesion to laminin and fibronectin, but not to collagens type I and IV. Reseeding of DLS with mESCs was inefficient (6.9 ± 0.5%), but was significantly enhanced (2.3 ± 0.1 fold) by pre-treating the scaffolds with media conditioned by A549 human lung adenocarcinoma cells, which we found to contain ~5 ?g/ml laminin. Furthermore, pre-treatment with A549-conditioned media resulted in a significantly more uniform distribution of the seeded mESCs throughout the engineered organ as compared to untreated DLS. Our study may advance whole lung engineering by stressing the importance of matching the integrin receptor repertoire of the seeded cells and the cell binding motifs of DLS. äEnhanced reseeding of decellularized rodent lungs with mouse embryonic(epmc).pdf DeepDyve Pubget Overpricing