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10.1186/s13148-016-0265-7 http://scihub22266oqcxt.onion/10.1186/s13148-016-0265-7 C5028999!5028999!27660665     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Clin+Epigenetics 2016 ; 8 (ä): ä Nephropedia Template TP {{tp|p=27660665|t=2016. Neonatal monocytes exhibit a unique histone modification landscape |pdf=|usr=}}{{27660665}} gab.com Text Neonatal monocytes exhibit a unique histone modification landscape JO: Clin Epigenetics http://www.kidney.de/pget.php?&tw=1&pmid=27660665 #FOAvip Background: Neonates have dampened expression of pro-inflammatory cytokines and difficulty clearing pathogens. This makes them uniquely susceptible to infections, but the factors regulating neonatal-specific immune responses are poorly understood. Epigenetics, including histone modifications, can activate or silence gene transcription by modulating chromatin structure and stability without affecting the DNA sequence itself and are potentially modifiable. Histone modifications are known to regulate immune cell differentiation and function in adults but have not been well studied in neonates. Results: To elucidate the role of histone modifications in neonatal immune function, we performed chromatin immunoprecipitation on mononuclear cells from 45 healthy neonates (gestational ages 23?40 weeks). As gestation approached term, there was increased activating H3K4me3 on the pro-inflammatory IL1B, IL6, IL12B, and TNF cytokine promoters (p?<?0.01) with no change in repressive H3K27me3, suggesting that these promoters in preterm neonates are less open and accessible to transcription factors than in term neonates. Chromatin immunoprecipitation with massively parallel DNA sequencing (ChIP-seq) was then performed to establish the H3K4me3, H3K9me3, H3K27me3, H3K4me1, H3K27ac, and H3K36me3 landscapes in neonatal and adult CD14+ monocytes. As development progressed from neonate to adult, monocytes lost the poised enhancer mark H3K4me1 and gained the activating mark H3K4me3, without a change in additional histone modifications. This decreased H3K4me3 abundance at immunologically important neonatal monocyte gene promoters, including CCR2, CD300C, ILF2, IL1B, and TNF was associated with reduced gene expression. Conclusions: These results provide evidence that neonatal immune cells exist in an epigenetic state that is distinctly different from adults and that this state contributes to neonatal-specific immune responses that leaves them particularly vulnerable to infections. Electronic supplementary material: The online version of this article (doi:10.1186/s13148-016-0265-7) contains supplementary material, which is available to authorized users. Twit Text FOAVip Neonatal monocytes exhibit a unique histone modification landscape ????Clin Epigenetics http://www.kidney.de/pget.php?&tw=1&pmid=27660665 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27660665 #FOAvip English Wikipedia <ref>{{Cite pmid|27660665}}</ref> Neonatal monocytes exhibit a unique histone modification landscape #MMPMID27660665 Bermick JR; Lambrecht NJ; denDekker AD; Kunkel SL; Lukacs NW; Hogaboam CM; Schaller MA Clin Epigenetics 2016[]; 8 (ä): ä PMID27660665show ga Background: Neonates have dampened expression of pro-inflammatory cytokines and difficulty clearing pathogens. This makes them uniquely susceptible to infections, but the factors regulating neonatal-specific immune responses are poorly understood. Epigenetics, including histone modifications, can activate or silence gene transcription by modulating chromatin structure and stability without affecting the DNA sequence itself and are potentially modifiable. Histone modifications are known to regulate immune cell differentiation and function in adults but have not been well studied in neonates. Results: To elucidate the role of histone modifications in neonatal immune function, we performed chromatin immunoprecipitation on mononuclear cells from 45 healthy neonates (gestational ages 23?40 weeks). As gestation approached term, there was increased activating H3K4me3 on the pro-inflammatory IL1B, IL6, IL12B, and TNF cytokine promoters (p? äNeonatal monocytes exhibit a unique histone modification landscape (epmc).pdf DeepDyve Pubget Overpricing