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10.1109/JSTQE.2016.2568160 http://scihub22266oqcxt.onion/10.1109/JSTQE.2016.2568160 C5028128!5028128!27660404     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       IEEE+J+Sel+Top+Quantum+Electron 2016 ; 22 (4): ä Nephropedia Template TP {{tp|p=27660404|t=2016. Single-Molecule Tracking and Its Application in Biomolecular Binding Detection |pdf=|usr=}}{{27660404}} gab.com Text Single-Molecule Tracking and Its Application in Biomolecular Binding Detection JO: IEEE J Sel Top Quantum Electron http://www.kidney.de/pget.php?&tw=1&pmid=27660404 #FOAvip In the past two decades significant advances have been made in single-molecule detection, which enables the direct observation of single biomolecules at work in real time and under physiological conditions. In particular, the development of single-molecule tracking (SMT) microscopy allows us to monitor the motion paths of individual biomolecules in living systems, unveiling the localization dynamics and transport modalities of the biomolecules that support the development of life. Beyond the capabilities of traditional camera-based tracking techniques, state-of-the-art SMT microscopies developed in recent years can record fluorescence lifetime while tracking a single molecule in the 3D space. This multiparameter detection capability can open the door to a wide range of investigations at the cellular or tissue level, including identification of molecular interaction hotspots and characterization of association/dissociation kinetics between molecules. In this review, we discuss various SMT techniques developed to date, with an emphasis on our recent development of the next generation 3D tracking system that not only achieves ultrahigh spatiotemporal resolution but also provides sufficient working depth suitable for live animal imaging. We also discuss the challenges that current SMT techniques are facing and the potential strategies to tackle those challenges. Twit Text FOAVip Single-Molecule Tracking and Its Application in Biomolecular Binding Detection ????IEEE J Sel Top Quantum Electron http://www.kidney.de/pget.php?&tw=1&pmid=27660404 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27660404 #FOAvip English Wikipedia <ref>{{Cite pmid|27660404}}</ref> Single-Molecule Tracking and Its Application in Biomolecular Binding Detection #MMPMID27660404 Liu C; Liu YL; Perillo EP; Dunn AK; Yeh HC IEEE J Sel Top Quantum Electron 2016[Jul]; 22 (4): ä PMID27660404show ga In the past two decades significant advances have been made in single-molecule detection, which enables the direct observation of single biomolecules at work in real time and under physiological conditions. In particular, the development of single-molecule tracking (SMT) microscopy allows us to monitor the motion paths of individual biomolecules in living systems, unveiling the localization dynamics and transport modalities of the biomolecules that support the development of life. Beyond the capabilities of traditional camera-based tracking techniques, state-of-the-art SMT microscopies developed in recent years can record fluorescence lifetime while tracking a single molecule in the 3D space. This multiparameter detection capability can open the door to a wide range of investigations at the cellular or tissue level, including identification of molecular interaction hotspots and characterization of association/dissociation kinetics between molecules. In this review, we discuss various SMT techniques developed to date, with an emphasis on our recent development of the next generation 3D tracking system that not only achieves ultrahigh spatiotemporal resolution but also provides sufficient working depth suitable for live animal imaging. We also discuss the challenges that current SMT techniques are facing and the potential strategies to tackle those challenges. äSingle-Molecule Tracking and Its Application in Biomolecular Binding D(epmc).pdf DeepDyve Pubget Overpricing