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10.1186/s12864-016-3084-5 http://scihub22266oqcxt.onion/10.1186/s12864-016-3084-5 C5027121!5027121!27640184     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       BMC+Genomics 2016 ; 17 (ä): ä Nephropedia Template TP {{tp|p=27640184|t=2016. Efficient generation and reversion of chromosomal translocations using CRISPR/Cas technology |pdf=|usr=}}{{27640184}} gab.com Text Efficient generation and reversion of chromosomal translocations using CRISPR/Cas technology JO: BMC Genomics http://www.kidney.de/pget.php?&tw=1&pmid=27640184 #FOAvip Background: Chromosomal translocations are a hallmark of cancer cells and give rise to fusion oncogenes. To gain insight into the mechanisms governing tumorigenesis, adequate model cell lines are required. Results: We employ the versatile CRISPR/Cas system to engineer cell lines in which chromosomal translocations are either generated de novo (CD74-ROS1) or existing translocations are reverted back to the original configuration (BCR-ABL1). To this end, we co-apply two guide RNAs to artificially generate two breakpoints and screen for spontaneous fusion events by PCR. Conclusions: The approach we use is efficient and delivers clones bearing translocationsin a predictable fashion. Detailed analysis suggests that the clones display no additional undesired alterations, implying that the approach is robust and precise. Electronic supplementary material: The online version of this article (doi:10.1186/s12864-016-3084-5) contains supplementary material, which is available to authorized users. Twit Text FOAVip Efficient generation and reversion of chromosomal translocations using CRISPR/Cas technology ????BMC Genomics http://www.kidney.de/pget.php?&tw=1&pmid=27640184 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27640184 #FOAvip English Wikipedia <ref>{{Cite pmid|27640184}}</ref> Efficient generation and reversion of chromosomal translocations using CRISPR/Cas technology #MMPMID27640184 Lekomtsev S; Aligianni S; Lapao A; Bürckstümmer T BMC Genomics 2016[]; 17 (ä): ä PMID27640184show ga Background: Chromosomal translocations are a hallmark of cancer cells and give rise to fusion oncogenes. To gain insight into the mechanisms governing tumorigenesis, adequate model cell lines are required. Results: We employ the versatile CRISPR/Cas system to engineer cell lines in which chromosomal translocations are either generated de novo (CD74-ROS1) or existing translocations are reverted back to the original configuration (BCR-ABL1). To this end, we co-apply two guide RNAs to artificially generate two breakpoints and screen for spontaneous fusion events by PCR. Conclusions: The approach we use is efficient and delivers clones bearing translocationsin a predictable fashion. Detailed analysis suggests that the clones display no additional undesired alterations, implying that the approach is robust and precise. Electronic supplementary material: The online version of this article (doi:10.1186/s12864-016-3084-5) contains supplementary material, which is available to authorized users. äEfficient generation and reversion of chromosomal translocations using(epmc).pdf DeepDyve Pubget Overpricing